Hai cercato: Purificazione proteine
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Reversed phase chromatography media, SOURCE™ 15RPC
Supplier: Cytiva
SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.
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Affinity coupling media, CNBr-activated Sepharose™ 4
Supplier: Cytiva
CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Affinity chromatography media, MabSelect SuRe™ LX
Supplier: Cytiva
MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.
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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Mass spectrometry reagents, MALDI matrices, single-use
Supplier: Thermo Fisher Scientific
MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.
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Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
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Ion exchange chromatography media, CM Sepharose™ Fast Flow
Supplier: Cytiva
CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
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Recombinant protein G, peroxidase conjugated, Calbiochem®
Supplier: Merck Millipore (Oncogene)
Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.
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BTSNM support materials, Chelex beads, 0,4 g with buffer
Supplier: G-Biosciences
Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
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Recombinant streptavidin
Supplier: G-Biosciences
Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.
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HCCA matrix
Supplier: BRUKER DALTRONIK
Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.
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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound
Supplier: Thermo Fisher Scientific
Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.
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Coupling catalyst, GlycoLink™
Supplier: Thermo Fisher Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Affinity chromatography media, Blue Sepharose™ High Performance
Supplier: Cytiva
This media is suitable for affinity chromatography.
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Affinity chromatography media, Glutathione Sepharose™ 4B
Supplier: Cytiva
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.
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Affinity chromatography media, Lentil Lectin Sepharose™ 4B
Supplier: Cytiva
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
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Affinity chromatography media, Ni Sepharose™ High Performance
Supplier: Cytiva
Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.
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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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TCEP, neutral, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
A ready-to-use stable, neutral form of the disulfide-reducing agent TCEP, hydrochloride.
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Pegylation reagent, MT(PEG)₄ methyl-PEG-thiol compound
Supplier: Thermo Fisher Scientific
Methyl-PEG4-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.
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Biacore consumables and accessories
Supplier: Cytiva
Biacore maintenance kits are convenient kits for proper instrument maintenance.
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Accessories and spare parts for ÄKTA start
Supplier: Cytiva
Bacinella assemblata Frac30
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Accessori e ricambi per ÄKTApilot
Supplier: Cytiva
Union, M6 femmina - 5/16" maschio
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Protein A from Staphylococcus aureus, MP Biomedicals
Supplier: MP Biomedicals
Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.