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82 risultati per Saggi proteici

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Saggi proteici

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

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Bradford protein assay kits (B-Pak)

Bradford protein assay kits (B-Pak)

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

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ReadiUse™ TCA Deproteinization Sample Preparation Kit

ReadiUse™ TCA Deproteinization Sample Preparation Kit

Supplier: AAT BIOQUEST

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.

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Lumiwox™ Acridinium NHS Ester

Lumiwox™ Acridinium NHS Ester

Supplier: AAT BIOQUEST

The acridinium NHS ester can be used to label proteins and nucleic acids.

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Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.

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GST protein interaction pull down kit, Pierce™

GST protein interaction pull down kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

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ReadiUse™ TCEP Removal Solution

ReadiUse™ TCEP Removal Solution

Supplier: AAT BIOQUEST

TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.

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Amplite® Rapid Colorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.

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Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Horseradish Peroxidase, Biotinylated, Pierce™

Horseradish Peroxidase, Biotinylated, Pierce™

Supplier: Thermo Fisher Scientific

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

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Phosphoprotein phosphate estimation assay Kit, Pierce™

Phosphoprotein phosphate estimation assay Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

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Amplite® Fluorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

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Protein Assay, Protn-Latex™

Protein Assay, Protn-Latex™

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.

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Micro BCA protein assay kit

Micro BCA protein assay kit

Supplier: Boster Bio

The Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve.

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Non-interfering Protein Assay™ kit, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

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Quantitative colorimetric peptide assay, Pierce™

Quantitative colorimetric peptide assay, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

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Glutathione Fluorometric Assay Kit

Glutathione Fluorometric Assay Kit

Supplier: Abcam

Simple assay to detect total glutathione.

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Streptavidin poly-HRP conjugate, Pierce™

Streptavidin poly-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

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Biotin labelling kit

Biotin labelling kit

Supplier: Abnova

Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

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Histone, from calf thymus, MP Biomedicals

Supplier: MP Biomedicals

Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

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2-D Quant kit

2-D Quant kit

Supplier: Cytiva

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

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Protein quantitation kits, Upti-Light

Protein quantitation kits, Upti-Light

Supplier: Uptima

The BCA method is very sensitive for protein detection. Peptide bonds and the four amino acids cysteine, cystine , tryptophan and tyrosine are relevant to the determination. The formation of Cu (I) ions by proteins under alkaline conditions corresponds to the biuret method of determination. A Cu (I ) ion is then bound by two molecules of BCA to a red colour complex. This complex can be detected very sensitively.

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Soluble Collagen Type I Solution, Bovine

Soluble Collagen Type I Solution, Bovine

Supplier: MARKET SOURCE PART PROCESS

Soluble bovine type I collagen acid extracted (Telocollagen) at 5 mg/ml. Soluble collagen can be used as a thin coating for 2D cell culture in any cell culture dish. For use in 3D cell culture, collagen hydrogels, and bioinks, sterile 20 ml bottle.

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