Auftragserfassung
Switzerland
ContactUsLinkComponent
 

Hai cercato: Saggi proteici

Saggi proteici

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Expand 4 Items
Caricamento...
VWR® Protein Precipitation Kit

VWR® Protein Precipitation Kit

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

Expand 1 Items
Caricamento...

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Supplier: ENZO LIFE SCIENCES

The only commercially available kit for isolation and enrichment of SUMOylated proteins.

Expand 1 Items
Caricamento...
Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

Expand 1 Items
Caricamento...

Thermal shift protein stability kits, GloMelt™

Supplier: Biotium

Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Expand 4 Items
Caricamento...
Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Expand 2 Items
Caricamento...

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Supplier: Tonbo Biosciences

Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry

Expand 1 Items
Caricamento...
Protein assay kits, RED 660™

Protein assay kits, RED 660™

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Expand 4 Items
Caricamento...

Protein carbonyl colorimetric assay kit

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

Expand 1 Items
Caricamento...
Conjugate stabilisers

Conjugate stabilisers

Supplier: Rockland Immunochemicals

Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

Expand 2 Items
Caricamento...

eFluxx-ID® multidrug resistance assay kit

Supplier: ENZO LIFE SCIENCES

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

Expand 2 Items
Caricamento...
Protein G Spin Antibody Purification Kit

Protein G Spin Antibody Purification Kit

Supplier: Abcam

Kit helps purify antibodies from serum, ascites and cell culture medium from various species.

Expand 1 Items
Caricamento...
Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)

Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)

Supplier: Abcam

Simplest assay to screen/study/characterize thioredoxin 1 inhibitors.

Expand 1 Items
Caricamento...
Saggio proteico Bradford, grado proteomico

Saggio proteico Bradford, grado proteomico

Supplier: VWR Chemicals

Il saggio proteico Bradford è il saggio colorimetrico migliore per quantificare la concentrazione proteica totale. Basato sulla formazione complessa tra residui basici e amminoacidi aromatici con colorante Coomassie® Brilliant Blue G-250, il metodo Bradford è più rapido, più semplice e più sensibile del metodo Lopwr.

Expand 1 Items
Caricamento...
PROTEOSTAT® protein aggregation assay

PROTEOSTAT® protein aggregation assay

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

Expand 1 Items
Caricamento...
Glycogen Assay Kit

Glycogen Assay Kit

Supplier: Cayman Chemical

A simple and sensitive tool for assaying glycogen from tissue.

Expand 1 Items
Caricamento...
Bicinchoninic acid (BCA) protein assay

Bicinchoninic acid (BCA) protein assay

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Expand 4 Items
Caricamento...
L-Lactate Assay Kit

L-Lactate Assay Kit

Supplier: Cayman Chemical

A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.

Expand 1 Items
Caricamento...
Micro Bicinchoninic Acid (BCA) protein assay

Micro Bicinchoninic Acid (BCA) protein assay

Supplier: G-Biosciences

The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Expand 2 Items
Caricamento...

Compatible Lowry protein assay

Supplier: G-Biosciences

These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

Expand 4 Items
Caricamento...

Osteoclast inhibitory lectin MAB MO XMO

Supplier: US Biological

Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.

Expand 1 Items
Caricamento...

PROTEOSTAT® protein refolding and aggregation sensing kit for microplates

Supplier: ENZO LIFE SCIENCES

ProteoStat® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye. 

Expand 1 Items
Caricamento...
Protein quantitation assay kits, CB™ Protein assay

Protein quantitation assay kits, CB™ Protein assay

Supplier: G-Biosciences

CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Expand 2 Items
Caricamento...
Protein quantitation assay kits, CB-X™ Protein assay

Protein quantitation assay kits, CB-X™ Protein assay

Supplier: G-Biosciences

CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

Expand 1 Items
Caricamento...
β-Hydroxybutyrate (Ketone Body) Assay Kit

β-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: Cayman Chemical

A sensitive tool for measuring β-HB levels in plasma, serum, or urine.

Expand 1 Items
Caricamento...
PROTEOSTAT® thermal shift stability assay kit

PROTEOSTAT® thermal shift stability assay kit

Supplier: ENZO LIFE SCIENCES

This kit has been designed for monitoring protein stability under systematic thermal stress conditions.

Expand 2 Items
Caricamento...
Solid phase biotinylation kits, EZ-Link™, Pierce™

Solid phase biotinylation kits, EZ-Link™, Pierce™

Supplier: Thermo Fisher Scientific

This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

Expand 3 Items
Caricamento...
Pierce™ Polyhis-Protein Interaction Pull Down Kit

Pierce™ Polyhis-Protein Interaction Pull Down Kit

Supplier: Thermo Fisher Scientific

The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.

Expand 1 Items
Caricamento...
Tetracysteine REASH Acetate *2 mM DMSO Solution*

Tetracysteine REASH Acetate *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

REASH acetate is a cell-permeable version of REASH, which is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.

Expand 1 Items
Caricamento...
Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent

Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent

Supplier: Thermo Fisher Scientific

The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Expand 1 Items
Caricamento...
Recommended for You