Hai cercato: Saggi enzimatici

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

Supplier: AAT BIOQUEST

Glucose, a monosaccharide, is the most important carbohydrate in biology.

Supplier: AAT BIOQUEST

Formaldehyde is a naturally occurring substance.

Supplier: AAT BIOQUEST

Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

Supplier: AAT BIOQUEST

Caspases play important roles in apoptosis and cell signaling.

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

Supplier: AAT BIOQUEST

The traditional BCA protein assay is widely used for quantifying proteins.

Supplier: AAT BIOQUEST

Site-selective modification of proteins is of major interest in chemical biology.

Supplier: AAT BIOQUEST

Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.

Supplier: AAT BIOQUEST

Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.

Supplier: AAT BIOQUEST

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).

Supplier: AAT BIOQUEST

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

Supplier: AAT BIOQUEST

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

Supplier: AAT BIOQUEST

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

Supplier: AAT BIOQUEST

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

Supplier: AAT BIOQUEST

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

Supplier: AAT BIOQUEST

A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.