You searched for: Saggi enzimatici
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric L-Lactate Assay Kit
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Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
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Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
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Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
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Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric Endotoxin Detection Kit
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Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
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Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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ReadiUse™ NADPH Regenerating Kit
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NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
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Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Colorimetric Tyrosinase Assay Kit
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Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
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Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
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The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Amplite® Colorimetric Lipase Activity Assay Kit
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Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
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Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*
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The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.
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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
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Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.
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Amplite® Fluorimetric Glucose and Sucrose Assay Kit
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Designed for consistent and dependable results. The Amplite® fluorimetric glucose and sucrose assay kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
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The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Fluorimetric Pyruvate Assay Kit
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Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
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Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.
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MMP-3 Green™ Substrate
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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Tyrosinase Assay Kit
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Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
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Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.
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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
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Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.
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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
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Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.
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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.