Saggi enzimatici
Sia che si misuri il consumo di substrato o la produzione complessiva, i saggi enzimatici vengono utilizzati per monitorare l'attività enzimatica. Se forniti dei materiali e dei reagenti necessari per qualsiasi metodo preferito, è possibile completare con successo studi di cinetica enzimatica e di inibizione. Mentre i metodi di campionamento continuo forniscono letture di attività durante tutto lo sviluppo, le tecniche discontinue calcolano l'attività dopo l'interruzione della reazione. I saggi enzimatici standardizzati forniranno un'identificazione e una misurazione accurate delle molecole proteiche presenti nei campioni.
Matrix Metalloproteinase-8 (MMP-8) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-8 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) using a thiopeptide as a chromogenic substrate.
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AEC Peroxidase substrate kit
Supplier: Enzo Life Sciences
The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.
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Matrix Metalloproteinase-3 (MMP-3) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-3 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96 well microplate format.
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Matrix Metalloproteinase-9 (MMP-9) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay SystemThe MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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Free Fatty Acid Fluorometric Assay Kit
Supplier: Cayman Chemical
A sensitive tool for measuring free fatty acids in plasma, serum, and urine.
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Fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.
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Protease Estimation Assay Kit, ProteSEEKER™
Supplier: G-Biosciences
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
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Matrix Metalloproteinase-3 (MMP-3) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-3 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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Calcineurin phosphatase assay kit
Supplier: Enzo Life Sciences
This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.
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Cathepsin K drug discovery kit
Supplier: Enzo Life Sciences
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.
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ProteoStat™ thioredoxin-1 assay kit for microplates
Supplier: Enzo Life Sciences
This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.
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Matrix Metalloproteinase-14 (MMP-14) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-14 Colorimetric Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a thiopeptide as a chromogenic substrate.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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Matrix Metalloproteinase-2 (MMP-2) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A Quantizyme™ Assay System. The MMP-2 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-2 (MMP-2, gelatinase A) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target.
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DPPIV Drug discovery kit
Supplier: Enzo Life Sciences
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.
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MMP inhibitor profiling kit
Supplier: Enzo Life Sciences
The MMP inhibitor profiling kit, fluorometric RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED.
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