Saggi enzimatici
Sia che si misuri il consumo di substrato o la produzione complessiva, i saggi enzimatici vengono utilizzati per monitorare l'attività enzimatica. Se forniti dei materiali e dei reagenti necessari per qualsiasi metodo preferito, è possibile completare con successo studi di cinetica enzimatica e di inibizione. Mentre i metodi di campionamento continuo forniscono letture di attività durante tutto lo sviluppo, le tecniche discontinue calcolano l'attività dopo l'interruzione della reazione. I saggi enzimatici standardizzati forniranno un'identificazione e una misurazione accurate delle molecole proteiche presenti nei campioni.
Fluorometric HTS assay kits, Caspase-3 and Caspase-8
Supplier: Biotium
Caspase-3 and Caspase-8 Fluorometric HTS Assay Kits provide a homogenous assay system for fast and highly sensitive detection of Caspase-3 or Caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.
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Caspase-1 assay kit for drug discovery
Supplier: Enzo Life Sciences
This kit is a complete assay system to measure protease activity of recombinant caspase-1. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm).
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MMP Fluorometric drug discovery kits, RED and GREEN
Supplier: Enzo Life Sciences
The matrix metalloproteinase (MMP) RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates OmniMMP™ RED or Fluorogenic Substrate (GREEN). The assays are performed in a convenient 96-well microplate format, with the compound NNGH also included as a prototypic control inhibitor.
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Complex IV Human Enzyme Activity Dipstick Assay Kit
Supplier: Abcam
Complex IV Human Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Complex IV Human Enzyme Activity in Cow, Human in Cell culture extracts, Tissue samples.
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Matrix Metalloproteinase-8 (MMP-8) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-8 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties
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HDAC/SIRT chemiluminescent drug discovery kit, CHEMILUM DE LYS®
Supplier: Enzo Life Sciences
The HDAC/SIRT chemiluminescent drug discovery kit is a complete assay system designed to measure histone deacetylase (HDAC) and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes.
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Ascorbate Assay Kit
Supplier: Cayman Chemical
A kit for measuring ascorbate.
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α-Amylase Inhibitor Screening Kit
Supplier: Abcam
Simple, 1-step assay for screening potential α-amylase inhibitors.
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Matrix Metalloproteinase-7 (MMP-7) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-7 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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PKA kinase activity kit
Supplier: Enzo Life Sciences
The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Protease and peptidase substrate
Supplier: Biotium
Fluorogenic substrates for proteases and peptidases.
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Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit
Supplier: Abcam
Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Enzyme Activity in Mouse, Rat, Cow, Human, Pig in Cell culture extracts, Tissue samples.
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Proteasome 20S Assay Kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.
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Superoxide dismutase assay kit
Supplier: Cayman Chemical
Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.
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Caspase-3 fluorometric assay kit
Supplier: Enzo Life Sciences
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
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Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-1 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) using a thiopeptide as a chromogenic substrate.
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck