Sie haben gesucht nach: Saggi cellulari
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Green detection kit, MITO-ID®
Supplier: Enzo Life Sciences
MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.
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NUCLEOLAR-ID® Green detection kit
Supplier: Enzo Life Sciences
Specifically designed for visualizing microscopically nucleoli in living cells.
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LDH Cytotoxicity Assay Kit
Supplier: Cayman Chemical
For measurement of LDH activity as an indicator of cell death.
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Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Supplier: Cayman Chemical
For rapid and easy determination of GSH in whole cells.
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Red long-term cell tracer, CYTO-ID®
Supplier: Enzo Life Sciences
CYTO-ID® is a live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.
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Hydrogen peroxide fluorometric detection kit
Supplier: Enzo Life Sciences
The Hydrogen Peroxide Fluorometric Detection Kit provides fast results in just 10 minutes for the measurement of either hydrogen peroxide or peroxidase activity in a convenient one-step homogeneous assay.
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LEADING LIGHT® Wnt reporter cell line
Supplier: Enzo Life Sciences
Wnt reporter cell line for discovering Wnt pathway modulators.
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Membrane potential detection kit, MITO-ID®
Supplier: Enzo Life Sciences
MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.
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CF® Dye Dextran 70000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.
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CF® Dye Dextran 10000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.
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CF® Dye Dextran 250000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.
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Live-dead cell staining kit
Supplier: Enzo Life Sciences
Kit utilises Live-dye™, a cell permeable green fluorescent dye (Ex(max): 488 nm; Em(max): 518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a red fluorescent dye (Ex(max): 488 nm; Em(max): 615 nm), which in viable cells is actively pumped out of the cytoplasm.
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NF[kappa]B transcription factor assay kits
Supplier: Rockland Immunochemicals
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
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ER-ID™ green assay kit
Supplier: Enzo Life Sciences
The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
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Apoptosis and necrosis quantitation kits
Supplier: Biotium
Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.
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Cellular senescence live cell analysis assay
Supplier: Enzo Life Sciences
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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ApoSENSOR™ ADP/ATP ratio assay kit
Supplier: Enzo Life Sciences
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
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CloneDetect
Supplier: Molecular Devices
Screen and select high value mammalian clones with label-free, in situ detection of IgG secretion with the ClonePix system, and other cell analysers with a green fluorescent channel.
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ROS-ID™ total ROS detection kit
Supplier: Enzo Life Sciences
This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.
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CF® Dye Dextran 3500 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.
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CYTO-ID® Autophagy detection kit
Supplier: Enzo Life Sciences
The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy and flow cytometry.
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LSD1 fluorometric drug discovery kit
Supplier: Enzo Life Sciences
The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.
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DPPIV/CD26 assay kit for biological samples
Supplier: Enzo Life Sciences
The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.
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Annexin V-FITC apoptosis detection kit
Supplier: Enzo Life Sciences
For the detection of apoptotic cells by flow cytometry.
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Annexin V-EGFP apoptosis detection kit
Supplier: Enzo Life Sciences
For the detection of apoptotic cells by flow cytometry.
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Neutrophil/Monocyte Respiratory Burst Assay Kit
Supplier: Cayman Chemical
Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
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TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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ApoSENSOR™ Cell viability assay kit
Supplier: Enzo Life Sciences
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
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Red hydrogen peroxide assay kit
Supplier: Enzo Life Sciences
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
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OXI-TEK TBARS assay kit
Supplier: Enzo Life Sciences
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.