Hai cercato: Saggi cellulari

Supplier: Merck Millipore (Novagen)

BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

Supplier: Merck Millipore (Oncogene)

Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

Supplier: Biotium

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

Supplier: OZ BIOSCIENCES

ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

Supplier: Abnova

Pyruvate Assay Kit is for quantitative colorimetric/fluorimetric pyruvate determination.

Supplier: Abnova

ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

Supplier: Abnova

Methylated DNA Capture Kit is used to enrich methylated DNA.

Supplier: MP Biomedicals

A rapid, simple and sensitive method for detecting mycoplasma contamination in cell cultures.

Supplier: Abnova

WST-1 cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model.

Supplier: Abnova

Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.

Supplier: Abnova

Nitric Oxide Assay Kit for the quantitative colorimetric determination of nitric oxide in cell, food sample, plasma, serum, tissue and urine.

Supplier: AAT BIOQUEST

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

Supplier: Biotium

This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.

Supplier: HIMEDIA

Saggi cellulari, EZAssay™ TBARS estimation kit for lipid peroxidation

Supplier: ENZO LIFE SCIENCES

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: CELL VIABILITY

The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.

Supplier: ENZO LIFE SCIENCES

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

Supplier: ENZO LIFE SCIENCES

Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

Yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® fluorimetric sodium ion quantification kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® fluorimetric lithium ion quantification kit uses our new robust lithium-ion indicator dye, Lithiumighty™ 520, which exhibits great fluorescence intensity enhancement upon binding to lithium ions.