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Saggi cellulari

Saggi cellulari

I saggi cellulari sono sempre stati uno strumento potente nel laboratorio di ricerca. Sono incredibilmente versatili e possono essere progettati per misurare praticamente qualsiasi funzione cellulare o biochimica. La rilevazione sensibile di sonde fluorescenti aiuta a valutare la segnalazione cellulare e le vie di morte cellulare, risultando utile per la rapida quantificazione della vitalità cellulare. I saggi cellulari possono essere utilizzati per misurare gli effetti di diversi trattamenti sulla vitalità cellulare e per valutare effetti tossici mediati da una varietà di meccanismi.

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S-Glutathionylated Protein Detection Kit

S-Glutathionylated Protein Detection Kit

Supplier: Cayman Chemical

A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.

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sPLA2 activity kit

Supplier: Enzo Life Sciences

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

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LEADING LIGHT® Wnt cell line medium pack

Supplier: Enzo Life Sciences

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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Cytoplasmic membrane labelling kits, CellBrite™

Cytoplasmic membrane labelling kits, CellBrite™

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

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Apoptosis inducer set

Apoptosis inducer set

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

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Neutrophil/Monocyte Respiratory Burst Assay Kit

Neutrophil/Monocyte Respiratory Burst Assay Kit

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

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p53 transcription factor assay kit

p53 transcription factor assay kit

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

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CV-Caspase 3 & 7 Detection kit

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

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HIF-1α transcription factor assay Kit

HIF-1α transcription factor assay Kit

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

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Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

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Autophagy detection tests, CYTO-ID®

Supplier: Enzo Life Sciences

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

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Auto-Ubiquitinylation Kit

Auto-Ubiquitinylation Kit

Supplier: Enzo Life Sciences

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

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Cellular senescence live cell analysis assay

Supplier: Enzo Life Sciences

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

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NUCLEAR-ID® green chromatin condensation detection kit

Supplier: Enzo Life Sciences

This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.

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Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9), Abcam

Supplier: Abcam

Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (ab267480) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.

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Black IHC chromogen (HRP), HIGHDEF®

Supplier: Enzo Life Sciences

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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