Hai cercato: Sistemi di associazione e marcatura delle proteine
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
Bungarotoxins and fluorescent bungarotoxin conjugates
Supplier: Biotium
Bungarotoxins are venom toxins from the many-banded krait (Bungarus Multicinctus), which inhibit neurotransmission at the neuromuscular junction. Alpha-bungarotoxin is a potent inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). Beta-bungarotoxin contains a small subunit that blocks voltage-gated K+ channels on the presynaptic membranes, and a large subunit that has phospholipase A2 activity. Fluorescent conjugates of alpha-bungarotoxin can be used to label acetylcholine receptors in skeletal muscle endplates.
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Antibody labelling kits, Mix-n-Stain™
Supplier: Biotium
Mix-n-Stain™ antibody labelling kits utilise a technology that allows rapid antibody labelling without a purification step.
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CF® Dye BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.
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CF®DI Aminooxy
Supplier: Biotium
CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.
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CF™ dye picolyl azide
Supplier: Biotium
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.
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Streptavidin conjugates
Supplier: Biotium
A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.
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HRP-conjugate stabiliser, SeramunStab® Stplus
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow or greenish, odourless, pH 7,3 to 7,5. Stabiliser for conjugates with monomeric HRP.
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Single-round RNA amplification and biotin labeling system
Supplier: ENZO LIFE SCIENCES
The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
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Nuclear-ID™ Blue/Green cell viability reagent
Supplier: ENZO LIFE SCIENCES
Nucleus dye
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Thiol-reactive dyes and reagents
Supplier: Biotium
MTS, TS and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.
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Biotin conjugation estimation, HOOK™ BiotinQuant
Supplier: G-Biosciences
HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.
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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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Tyramide amplification kit with HRP goat, anti-mouse and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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CF® Dye Azide
Supplier: Biotium
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
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Carbohydrate coupling resin
Supplier: G-Biosciences
Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.
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Nuclear-ID™ red/green cell viability reagent
Supplier: ENZO LIFE SCIENCES
Cell viability dye
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Chemiluminescent labelling kit
Supplier: ENZO LIFE SCIENCES
A complete kit for the labeling of antibodies, proteins, peptides, and nucleic acids with Acridinium NHS Ester.
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Aqua 431 dUTP
Supplier: ENZO LIFE SCIENCES
Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua 431 dUTP can also be used for multicolor fluorescence labelling.
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Red 580 dUTP
Supplier: ENZO LIFE SCIENCES
Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.
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Affibody® Imaging Agent, Negative Control
Supplier: Abcam
Product to be used as a negative control for ErbB 2 Affibody® Molecule Imaging Agent.
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Reactive biotin compounds
Supplier: Biotium
Biotium offers reactive biotin compounds with a variety of reactive functional groups.
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Amine reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ d-Biotin (Vitamin H), 500 mg
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Annexin V conjugates
Supplier: Biotium
Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
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Phalloidin conjugates
Supplier: Biotium
Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.
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CF® Dye hydrazide
Supplier: Biotium
Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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Antibody labelling kits, Mix-n-Stain™ Biotin
Supplier: Biotium
Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.
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Stabiliser for calibrators and controls, SeramunStab® STD
Supplier: Apollo Scientific
Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.
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Stabiliser for sensitive biomolecules, SeramunStab® STB
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.