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Sistemi di associazione e marcatura delle proteine

Sistemi di associazione e marcatura delle proteine

I sistemi di coniugazione e marcatura delle proteine offrono un mezzo pratico per collegare marcatori fluorescenti o biotine ad anticorpi e proteine più grandi. Questi kit sono ideali per molte applicazioni, tra cui citometria a flusso, microscopia a fluorescenza, immunoistochimica, rilevamento primario, ELISA, immunocitochimica e FISH. Questi sistemi sono disponibili con una varietà di coloranti, biotina, agenti fluorescenti e tutti i componenti necessari per eseguire più coniugazioni e purificazioni. La marcatura delle proteine segue un protocollo semplice: reazione, separazione e quindi utilizzo.

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SeramunGelb slow

Supplier: Apollo Scientific

SeramunGelb slow

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SeramunPurple prec

Supplier: Apollo Scientific

SeramunPurple prec

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SeramunBlau spot dark

Supplier: Apollo Scientific

SeramunBlau spot dark

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AP-conjugate stabiliser, SeramunStab® STAP

Supplier: Apollo Scientific

Liquid product colourless odourless pH-value 5,9 to 6,1, Stabiliser for very low concentrated AP conjugates. Suitable for proteins resistant to pH values <6,0.

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AP-conjugate stabiliser, SeramunStab® STAP2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,5 to 7,7. Stabiliser for a broad range of AP conjugates for various assay conditions. Useful for antibodies sensitive to pH <7,0.

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SeramunBlock NP

Supplier: Apollo Scientific

Protein free blocking solution, 5X concentrated ready-to-use solution. For preservation and stabilisation of proteins on different surfaces.

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SeramunBlock

Supplier: Apollo Scientific

Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

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SeramunBlau fast2

Supplier: Apollo Scientific

Fast reacting, ready to use, Horseradish peroxidase (HRP) substrate solution used to enable high sensitivies in ELISA testing within 20 minutes for in vitro sysytems.

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SeramunBlau prec

Supplier: Apollo Scientific

SeramunBlau prec

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Thiol-reactive dyes and reagents

Thiol-reactive dyes and reagents

Supplier: Biotium

MTS, TS and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.

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Dye conjugates dUTP CF®

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Lipophilic carbocyanine dyes and CellBrite cytoplasmic membrane labelling kits

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

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D-luciferin luminescent substrates

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

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Reactive dye conjugates

Supplier: Biotium

Reactive dyes containing an amine functional group.

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Avidin, biotin, protein A/G and streptavidin reagents

Avidin, biotin, protein A/G and streptavidin reagents

Supplier: Rockland Immunochemicals

Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

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