Estrazione delle proteine

Supplier: G-Biosciences

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Supplier: Enzo Life Sciences

Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.

Supplier: G-Biosciences

FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

Supplier: G-Biosciences

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Supplier: Abcam

Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

Supplier: Abcam

Lipid Extraction Kit (Chloroform-Free) provides a safer, chloroform-free method for efficient lipid isolation from plasma, serum, tissue, and cells. The recovered organic phase can be dried and resuspended for downstream lipid analysis. - Cited in over 15 publications - Supports multiple sample types including - plasma, serum, tissue, suspension cells, and adherent cells. - Chloroform-free - eliminates the use of toxic chloroform in lipid extraction - Efficient lipid isolation – high-yield lipid extraction with minimal contamination - Sufficient for 50 lipid preparations (25 µL sample size)

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).

Supplier: Thermo Fisher Scientific

The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Supplier: Abcam

Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations. - Cited in over 65 publications - Isolates mitochondria from both hard and soft tissues, such as brain, liver, and heart - Suitable for western blotting, immunocapture, or activity assays - Simple Protocol – no need for ultracentrifugation, making the process more efficient and user-friendly

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

Supplier: Abcam

Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays. - Cited in over 75 publications - Isolates mitochondria from cultured mammalian cells with ease - Useful in Western blotting, immunocapture, or mitochondrial activity assays - Sufficient reagents for up to 20 benchtop mitochondria isolations

Supplier: Abcam

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.