Soluzioni tampone
Resistendo alle variazioni di pH, i tamponi proteggono i campioni quando vengono aggiunti acidi o basi. Disponibili in polvere e in forma liquida, tracciano efficacemente le proteine durante l'isolamento e la separazione tramite elettroforesi. I tamponi salati possono anche essere utilizzati per calibrare e standardizzare i pHmetri per ottenere letture accurate. Le sostanze bloccanti impediscono l'adesione degli anticorpi ai siti liberi dalla membrana, che produrrebbero un background interferente. In varie concentrazioni per soddisfare qualsiasi esigenza, i tamponi possono essere disponibili in formato liquido o disidratato.
TRIS-buffered saline (TBS, 20X), with 2% Tween 20
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 20X), with 2% Tween 20
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Phosphate, 0,2 M buffer solution, pH 7,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, Valore pH: 7,5, Liquid
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Laemmli SDS sample buffer, reducing (6X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, reducing, 6X
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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Laemmli SDS sample buffer with pyronin Y, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, with PyroninY, 4X
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MES-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MES-SDS running buffer (20X)
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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TES, 0,2 M buffer soln., pH 7,5
Supplier: Thermo Fisher Scientific
TES buffer solution, 0,2 M, Valore pH: 7,5, Liquid
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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TRIS-buffered saline (TBS, 10X) pH 8,0
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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TBE running buffer (10X)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE running buffer (10X)
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NP-40 lysis buffer (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer (2X), Thermo Fisher Scientific
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Tween 20 washing buffer, 2% in PBS (20X)
Supplier: Thermo Fisher Scientific
Washing buffer, Tween 20 washing buffer, 2% in PBS (20X)
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IEF Cathode buffer (10X) with lysine and arginine
Supplier: Thermo Fisher Scientific
Running Buffer, IEF Cathode buffer (10X) with lysine and arginine
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