Soluzioni tampone

Resistendo alle variazioni di pH, i tamponi proteggono i campioni quando vengono aggiunti acidi o basi. Disponibili in polvere e in forma liquida, tracciano efficacemente le proteine durante l'isolamento e la separazione tramite elettroforesi. I tamponi salati possono anche essere utilizzati per calibrare e standardizzare i pHmetri per ottenere letture accurate. Le sostanze bloccanti impediscono l'adesione degli anticorpi ai siti liberi dalla membrana, che produrrebbero un background interferente. In varie concentrazioni per soddisfare qualsiasi esigenza, i tamponi possono essere disponibili in formato liquido o disidratato.

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Citrate-buffered saline (20X)

Supplier: Thermo Fisher Scientific

Saline-sodium citrate (SSC) buffer.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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CHES, 0.5 M buffer solutions

Supplier: Thermo Fisher Scientific

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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Blocking reagents, Blotto

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP Biomedicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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BIS-TRIS, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

BIS-TRIS buffer solution, 0,2 M, Valore pH: 6,5, Colourless, Odourless, Liquid

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Tris base buffer, powder, Corning®

Supplier: Corning

Tris is commonly used in buffer solutions in biochemical and molecular biological research.

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Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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TE buffer, 1X, liquid, Corning®

Supplier: Corning

Tris-EDTA (TE) buffer is commonly used in work with nucleic acids.

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Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.