You searched for: Kit e reagenti per la purificazione degli acidi nucleici

Supplier: VWR Chemicals

Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.

Supplier: VWR Chemicals

Bacterial DNA Mini Kit for the extraction of high quality DNA from gram-positive and gram-negative bacteria. For up to 1×10⁹ cells per preparation typical yield is up to 30 µg.

Supplier: VWR Chemicals

Plant RNA Kit efficiently isolates RNA from a wide range of plant species and plant tissue, effectively removing genomic DNA, polysaccharides, phenol and other secondary metabolites. Starting amount can be up to 100 mg plant tissue. The isolated RNA is directly suitable for downstream applications.

Supplier: VWR Chemicals

Easy to use method for isolation up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues <1×10⁷ cells or 30 mg tissue.

Supplier: VWR Chemicals

The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

Supplier: VWR Chemicals

Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.

Supplier: VWR Chemicals

Fast, easy, and economical kit for isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results.

Supplier: VWR Chemicals

Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

Supplier: VWR Chemicals

The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.

Supplier: VWR Chemicals

Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal sample with organic solvents using spin columns.

Supplier: OMEGA BIO-TEK

Il kit E-Z 96® Total RNA è ideato per isolare l’RNA cellulare totale da massimo 5×10⁶ cellule in coltura o tessuti morbidi. Questo kit consente di processare campioni singoli o multipli in meno di 60 minuti. Utilizzando la tecnologia con piastra di silice HiBind®, si elimina la necessità di eseguire estrazioni con fenolo/cloroformio, ultracentrifugazione a gradiente CsCl e precipitazione con isopropanolo o LiCl. I campioni vengono sottoposti a lisi in un tampone di lisi denaturante che rende inattivi gli RNasi. Le condizioni di legame vengono regolate e il lisato viene trasferito in una piastra a 96 pozzetti HiBind® RNA, dove l’RNA viene purificato con tre cicli di lavaggio. L’RNA di alta qualità viene eluito in acqua priva di RNasi. L’RNA purificato con il metodo E-Z 96® Total RNA è pronto per applicazioni quali RT-PCR, qPCR, visualizzazione differenziale, microsaggi e altre applicazioni downstream.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.

Supplier: OMEGA BIO-TEK

Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.

Supplier: OMEGA BIO-TEK

Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.

Supplier: AGILENT

The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2 to 3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12 to 48 hours.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0,1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.

Supplier: Merck

BacterialXpress™ Nucleic acid extraction kit is intended for the purification of bacterial DNA from plasma, serum, cerebral spinal fluid, amniotic fluid, tissue, bone marrow, and cell culture.

Supplier: OMEGA BIO-TEK

The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500 to 4000 µl plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or with automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.

Supplier: VWR Chemicals

TriFast™ is a complete ready to use reagent for simultaneous isolation of RNA, DNA and proteins from liquid samples, tissue and cells.

Supplier: VWR Chemicals

Dependable isolation of high quality total cellular DNA from a variety of plant species and tissues. peqGOLD homogeniser columns remove cell debris and improve sample handling following lysis.

Supplier: VWR Chemicals

Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Blood DNA Kit consente di isolare rapidamente DNA totale da 250 µl di sangue intero trattato con anticoagulanti congelato o appena prelevato e può essere utilizzato inoltre per la preparazione di DNA genomico da buffy coat, siero, plasma, midollo osseo, linfociti, piastrine e liquidi organici. Il kit consente di processare contemporaneamente campioni singoli o multipli in meno di 30 minuti e di eliminare le estrazioni con fenolo/cloroformio e passaggi lunghi quali precipitazioni con isopropanolo o etanolo. Il DNA purificato con il metodo E.Z.N.A.® Blood DNA è pronto per applicazioni quali PCR, Southern blotting o digestione con enzimi di restrizione.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

Isolate total RNA from cells or soft tissues using spin column.

Supplier: OMEGA BIO-TEK

Il kit E.Z.N.A.® Tissue DNA consente di isolare in modo semplice, rapido ed economicamente conveniente il DNA da campioni di origini diverse, tra cui cellule e tessuti animali congelati o appena prelevati. Dopo la lisi cellulare, il processo di purificazione del DNA può essere completato in meno di 30 minuti. Grazie al protocollo E.Z.N.A.® Tissue DNA, è possibile processare in qualsiasi momento e senza difficoltà fino a 30 mg di tessuto. Con il kit a colonnina da centrifuga è possibile processare un singolo campione o più campioni contemporaneamente. L'utilizzo del kit consente di eliminare le estrazioni con fenolo/cloroformio o passaggi lunghi quali precipitazioni con isopropanolo o etanolo. Il DNA purificato utilizzando il kit E.Z.N.A.® Tissue DNA è pronto per la maggior parte delle applicazioni downstream, quali PCR, southern blotting e digestione con enzimi di restrizione.

Supplier: OMEGA BIO-TEK

Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

Supplier: OMEGA BIO-TEK

Il kit E.Z.N.A.® MicroElute Total RNA fornisce un metodo rapido e facile per isolare fino a 50 µg di RNA totale da piccole quantità di cellule eucariotiche in coltura, tessuti quali campioni dissezionati a laser (LDS) o aspirati con ago fine (FNA). Normalmente, in un unico esperimento è possibile utilizzare fino a 5×10⁵ cellule eucariotiche o 5 mg di tessuto, a seconda del tipo di tessuto utilizzato. Questo kit consente di processare campioni singoli o multipli in meno di 30 minuti. Non si richiedono estrazioni con fenolo/cloroformio e passaggi lunghi quali ultracentrifugazione a gradiente di CsCl e precipitazione con isopropanolo o LiCl. L’RNA purificato può essere eluito con 10 - 15 µl di acqua esente da nucleasi. L’RNA purificato è adatto per la maggior parte di applicazioni downstream quali RT-PCR, northern blotting, purificazione Poly A+, saggi di protezione da nucleasi e traduzione in vitro.

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.