Reagenti per RT-PCR e cDNA

Supplier: Enzo Life Sciences

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

Supplier: AGILENT

AccuScript High-Fidelity Reverse Transcriptase delivers the highest RT accuracy currently available.

Supplier: Quantabio

I kit qScript™ cDNA Synthesis contengono qScript™ Reverse Transcriptase, una miscela di MMLV ingegnerizzata e proteina per l'inibizione della ribonucleasi, ottimizzata per una sintesi di cDNA sensibile e affidabile. I reagenti per la sintesi di cDNA sono forniti in diversi formati supermix e in kit per soddisfare le esigenze specifiche delle singole applicazioni.

Supplier: Enzo Life Sciences

AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

Supplier: Quantabio

La One-Step Kit RT-PCR qScript ™ XLT è un sistema conveniente e altamente sensibile per l'amplificazione di RNA modelli fino a 2 kb. sintesi del cDNA e l'amplificazione PCR vengono effettuate nello stesso tubo senza aprire tra procedure. Questo sistema è stato ottimizzato per garantire la massima RT-PCR efficienza, sensibilità e specificità.

Supplier: AGILENT

Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Supplier: Abcam

cDNA Kit ab286905 (previously known as Novo cDNA Kit M1165-100) contains A Moloney-Murine Leukemia Virus Reverse Transcriptase With Genetic Modifications To Abolish Rnase H Activity To Achieve Thermal Stability.

Supplier: Quantabio

Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Supplier: OriGene

qSTAR first-strand cDNA synthesis kit for miRNA is a two-step protocol kit.

Supplier: Thermo Fisher Scientific

The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Supplier: Thermo Fisher Scientific

The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Supplier: Thermo Fisher Scientific

RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Supplier: Thermo Fisher Scientific

The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

Supplier: Thermo Fisher Scientific

Maxima™ Reverse Transcriptase is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Supplier: Thermo Fisher Scientific

RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.