Hai cercato: Enzimi e kit qPCR/RT-qPCR

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® è un cocktail pronto all'uso, con concentrazione 5X, per PCR real-time (qPCR) che vince molti inibitori della PCR noti, spesso presenti nei campioni grezzi estratti dall'ambiente e da tessuti vegetali o animali. Contiene tutti i componenti, ad eccezione di primer, sonde e templato. Il ToughMix® cinque volte concentrato consente l'aggiunta di maggiori quantità di templato e il miglioramento della sensibilità su campioni a bassa concentrazione. PerfeCta® MultiPlex qPCR ToughMix® è stato ottimizzato per fornire la massima efficienza, sensibilità e specificità della PCR in volumi di reazione ridotti con protocolli PCR rapidi o convenzionali.

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Supplier: Quantabio

PERFECTA® qPCR ToughMix® è un 2X concentrato pronto per l'uso di cocktail di reazione per l'amplificazione PCR di modelli di DNA che supera molti noti inibitori del PCR, spesso presenti nei campioni di greggio estratto da campioni ambientali, tessuti vegetali, o tessuti animali. E 'un Real-Time qPCR reagente versatile e robusta che garantisce la massima sensibilità ed efficienza PCR con una varietà di sonde chimiche fluorogeniche, tra cui le sonde di idrolisi TaqMan®. PERFECTA® qPCR ToughMix® contiene tutti i componenti necessari di reazione, ad eccezione di primer, sonde (s) e il modello di DNA. Il colore azzurro del colorante tracciante AccuVue ™ semplifica il montaggio reazione in bianco piatti, o chiari e aiuta a minimizzare pipettando o errori di miscelazione. Non interferisce con le prestazioni qPCR o influenzare la stabilità del prodotto.

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® è una miscela pronta per l'uso e altamente sensibile per la PCR quantitativa con precedente reazione di trascrittasi inversa (RT-qPCR) di templati di RNA.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

Supplier: Quantabio

La miscela SuperMix con concentrazione 2X è un assortimento di reazioni pronte all'uso e contiene tutti i componenti necessari eccetto i primer, le sonde e i templati. Questo sistema consente di superare i molteplici limiti delle miscele Master per PCR tradizionali, consentendo l'amplificazione non affetta da errore sistematico di fino a cinque sequenze target in un'unica provetta. L'occultamento di ampliconi a basso numero di copie da parte di target di riferimento con numero di copie più elevato durante l'amplificazione è un problema comune nella PCR multiplex. Ciò può deviare o mascherare la rappresentazione e la quantificazione delle sequenze target a basso numero di copie. PerfeCTa® MultiPlex qPCR SuperMix offre una' elevata sensibilità paragonabile a quelle dei saggi mediante sonda in qPCR singleplex senza dover limitare o variare le concentrazioni dei primer.

Supplier: Thermo Fisher Scientific

The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: Thermo Fisher Scientific

Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Supplier: AAT BIOQUEST

ROX dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT BIOQUEST

TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT BIOQUEST

A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT BIOQUEST

JUN dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.