Hai cercato: Enzimi e kit qPCR/RT-qPCR

Supplier: Quantabio

La sintesi di cDNA e l'amplificazione PCR vengono eseguite nella stessa provetta senza necessità di continue aperture. Questi kit sono ottimizzati per consentire massima efficienza e specificità della RT-PCR e consentono coamplificazione non affetta da errore sistematico nelle trascrizioni di RNA target a basso numero di copie in presenza di geni di riferimento presenti con numero di copie più elevato. Pertanto, i kit qScript™ One-Step qRT-PCR offrono una migliore sensibilità rispetto agli altri reagenti qPCR reperibili in commercio. La miscela qScript™ One-Step Master Mix massimizza le attività sia della trascrittasi inversa che degli enzimi della PCR, riducendo al minimo la formazione di dimeri di primer. L'alta specificità è assicurata dalla Taq DNA polimerasi AccuStart™ legata da anticorpi monoclonali. Al raggiungimento della temperatura di 95 °C, gli anticorpi denaturano irreversibilmente rilasciando la Taq DNA polimerasi completamente attiva e non modificata. I kit qScript™ One-Step qRT-PCR sono disponibili per tutti gli strumenti per PCR Real time, comprese quelle che richiedono la normalizzazione mediante colorante di riferimento ROX.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Salmonella spp. in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Salmonella spp.

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

Le miscele PerfeCTa® qPCR SuperMixes con concentrazione 2X sono assortimenti di reazioni pronte all'uso e contengono tutti i componenti necessari eccetto i primer, la sonda e il templato per qPCR.

Supplier: VWR Chemicals

VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: AGILENT

Increases specificity and yield of primary PCR amplification products.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via hydrolysis probes. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: AGILENT

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.

Supplier: Quantabio

FastMixes include tamponi e stabilizzatori ottimizzati per la massima efficienza, riproducibilità e sensibilità della PCR con sonde TaqMan® e TaqMan® MGB. Una componente chiave delle miscele PerfeCTa® qPCR FastMixes è la Taq DNA polimerasi AccuFast™ che consente un'attivazione istantanea e una cinetica per una polimerizzazione rapida. Durante l'attivazione termica (20 secondi a 95°C), gli anticorpi denaturano irreversibilmente rilasciando la Taq DNA polimerasi completamente attiva e non modificata. Ciò promuove l'estensione specifica ed efficace del primer con la comodità di una fase di preparazione della reazione a temperatura ambiente. Le miscele FastMixes assicurano massima efficienza, sensibilità e specificità della PCR e un robusto segnale fluorescente con reazioni PCR standard o rapide.

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR master mix kits with high/low Rox offer superior sensitivity for improved quantification and reproducibility.

Supplier: AGILENT

MVP RNA is application-ready RNA available in small economical pack sizes.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMix e FastMix™ consentono una PCR quantitativa efficiente, sensibile e precisa con tamponi proprietari e stabilizzatori SYBR® Green che massimizzano il segnale in fluorescenza, l'efficienza della PCR e la riduzione di dimeri di primer. Questi SuperMix e FastMix™ sono stati ottimizzati per tutti gli strumenti di PCR real time, inclusi quelli che richiedono la normalizzazione con colorante di riferimento ROX o fluoresceina.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).