Hai cercato: Supplementi ed additivi per colture cellulari

Supplier: Corning

Il supplemento per terreni di crescita Corning® Nu-Serum™ con una concentrazione proteica ridotta offre un'alternativa ai sieri di vitelli appena nati, sieri bovini fetali ed altri sieri che vengono normalmente utilizzati per la coltura cellulare. La ridotta concentrazione proteica facilita la purificazione delle proteine, la produzione virale, la produzione di anticorpi monoclonali e lo screening ed aumenta la frequenza della trasfezione corretta di cellule.

Supplier: Thermo Fisher Scientific

A 1X stock solution contains 500 μM AEBSF.HCl, 150 nM aprotinin, 1 μM E-64, 0,5 mM EDTA and 1 μM leupeptin hemisulfate. Reconstitute each vial with 1 ml water to obtain a 100X stock solution.

Supplier: Corning

Questo collante cellulare e tissutale Corning® Cell-Tak™ è una formulazione di proteine polifenoliche da Mytilus edulis (mitile marino). Queste proteine sono i componenti chiave della colla secreta dal mitile per ancorarsi a substrati solidi nell'ambiente marino. Il prodotto Corning® Cell-Tak™ viene utilizzato per ottenere l'aderenza di cellule o sezioni tissutali a svariati tipi di superfici, inclusi plastica, vetro, metallo, PTFE e materiali biologici. Può semplificare la manipolazione dei campioni biologici per numerose tecniche in vitro, inclusi ibridazione in situ, immunodosaggi, microiniezione e immunoistochimica, nonché l'adesione di cellule primarie nella coltura. Il prodotto Corning® Cell-Tak™ è biocompatibile e non è specie specifico.

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

Supplier: Corning

Synthemax® II-SC Substrate is an easy to use, self-coating material for creating a unique synthetic surface that mimics the natural cell environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and directs differentiation into specialised cell types. In addition, it offers a synthetic, xeno-free alternative to biological coatings and/or feeder cell layers used in hES and iPS cell culture, whenever needed and appropriate.

Supplier: Corning

HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells

Supplier: Corning

96-well plates, coated, PS non-sterile, flat bottom, Corning® Matrigel®

Supplier: MP Biomedicals

Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.

Supplier: Cytiva

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

Supplier: MP Biomedicals

Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine

Supplier: MP Biomedicals

Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.

Supplier: MP Biomedicals

A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

Supplier: Cytiva

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

Supplier: US Biological

Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).

Supplier: Tonbo Biosciences

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

Supplier: FACELLITATE

The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.

Supplier: MP Biomedicals

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

Supplier: MP Biomedicals

Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

Supplier: MP Biomedicals

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.