Hai cercato: Supplementi ed additivi per colture cellulari

Supplier: VWR Chemicals

Parte idrosolubile di lievito autolisato contenente una fonte di complesso di vitamina B.

Supplier: VWR Chemicals

Egg yolk tellurite emulsion, VWR®

Supplier: VWR Chemicals

Contiene una miscela di inibitori della proteasi: AEBSF, Aprotinina, E 64, Bestatina e Leupeptina. Ogni fiala può essere ricostituita in 1 ml di acqua deionizzata per preparare 100X di soluzione.

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® nephrotoxicity library is a focused collection of 86 compounds with defined and diverse nephrotoxicity, including analgesic nephropathy, interstitial nephritis, glomerularopathy, proximal and distal tubulopathy, acute renal failure, chronic kidney disease, and more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. Most of the compounds are dissolved in DMSO at 10 mM unless otherwise stated in the documentation provided with the library. Selected compounds are dissolved in DI water. All compounds are provided at 100 μl volumes in a 96-well plate. This library is an essential cost-effective tool for predictive toxicology screening and assay development.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.

Supplier: Thermo Fisher Scientific

Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

Supplier: G-Biosciences

The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.

Supplier: PROTIDE PHARMACEUTICALS

Material grade - USP, Ph. Eur.

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2 °C to 8 °C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks.
After thawing the passage kit can be stored in the dark at 2 °C to 8 °C for up to 1 month. Do not heat the kit over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances).
Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.
Overtrypsination causes irreversible damage.
Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

Supplier: PROVITRO

The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: PROTIDE PHARMACEUTICALS

General serum replacement for many cell types from a variety of species. TCM™ is a defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. TCM™ contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® histaminergic and melatonin ligands plate contains 41 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The Histaminergic and Melatonin Ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® hepatotoxicity library is a focused collection of 238 compounds with defined and diverse hepatotoxicity, including steatosis, mitochondrial toxicity, Mallory body formation, cholestatic effects, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.

Supplier: Biowest

Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

Supplier: VWR Chemicals

Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: ENZO LIFE SCIENCES

Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: G-Biosciences

Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.