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Reagenti per trasfezione

Avantor offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. While considering a suitable transfection reagent, it is important to identify the cell type and culture conditions. Specialized transfection reagents will address rare cell cultures, neurons, and primary cells are more difficult to manipulate for porous characteristics. Avantor also offers products for electroporation and the consumables needed to support the transfection workflow.

siRNA transfection reagent, RiboJuice™

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

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DNA kit, BacMagic™-2

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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Transfection kit, BacMagic™

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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PULSin® Protein Delivery Reagents

PULSin® Protein Delivery Reagents

Supplier: SARTORIUS

PULSin® protein delivery reagent is dedicated to the delivery of proteins, antibodies and peptides into cells and offers a powerful approach to functional studies. PULSin® can be used to target intracellular proteins with antibodies in living cells without fixation.

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DNA transfection reagent for virus production, PEIpro®

DNA transfection reagent for virus production, PEIpro®

Supplier: Polyplus-transfection

PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus and recombinant proteins by Transient Gene Expression (TGE) in suspension-adapted mammalian cell lines cultivated in shaker flasks, platform shakers or stirred tank bioreactors.

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INTERFERin® siRNA and miRNA Transfection Reagents

INTERFERin® siRNA and miRNA Transfection Reagents

Supplier: SARTORIUS

INTERFERin® is a powerful, ready to use siRNA and miRNA reagent which provides more than 90% silencing efficiency at 1 nM siRNA in a wide variety of cells.

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jetPRIME® DNA and siRNA Transfection Reagents

jetPRIME® DNA and siRNA Transfection Reagents

Supplier: SARTORIUS

The jetPRIME® DNA and/or siRNA transfection reagent is a powerful and versatile reagent designed to ensure high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells.

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DNA/siRNA delivery reagent, in vivo-jetPEI®

DNA/siRNA delivery reagent, in vivo-jetPEI®

Supplier: Polyplus-transfection

in vivo-jetPEI® reagent provides versatile, reproducible and reliable delivery of all types of nucleic acids (DNA, siRNA, miRNA and more) in animal models, for functional studies and RNA interference experiments using various administration routes.

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Transfection reagent diluent, 150 mM NaCl solution

Transfection reagent diluent, 150 mM NaCl solution

Supplier: Polyplus-transfection

This 150 mM NaCl solution is recommended as dilution reagent for DNA transfection with jetPEI®.

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jetPEI® HTS DNA Transfection Reagents

jetPEI® HTS DNA Transfection Reagents

Supplier: SARTORIUS

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery, perfectly suited for High Throughput Screening applications (HTS).

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Transduction enhancer, in vivo ViroMag™

Transduction enhancer, in vivo ViroMag™

Supplier: OZ BIOSCIENCES

In vivo ViroMag™ transfection reagent has been designed for in vivo targeted transduction with any viral vector. Virus/nanoparticle complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

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Transduction enhancer, AdenoMag™

Transduction enhancer, AdenoMag™

Supplier: OZ BIOSCIENCES

AdenoMag™ transfection reagent is a magnetic nanoparticles formulation specifically dedicated to enhance Adenovirus and Adeno Associated Virus (AAV) infection and transduction capacities. Based on Magnetofection™ technology, it allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses.

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Transduction reagent, ViroMag™

Supplier: OZ BIOSCIENCES

ViroMag™ transduction reagent is a magnetic nanoparticles formulation dedicated to increase virus infection and transduction capacities. Based on Magnetofection™ technology, this reagent allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus transduction with extremely low vector doses. Due to its specific properties, ViroMag™ is ideal to infect non permissive cells.

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Transduction reagent, ViroMag™ R/L

Transduction reagent, ViroMag™ R/L

Supplier: OZ BIOSCIENCES

ViroMag™ R/L transduction reagent is a magnetic nanoparticles formulation optimised for rRetroviruses and lentiviruses. Based on Magnetofection™ technology, this reagent allows concentrating the complete applied dose of retro/lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses.

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Transduction reagent, Viro-MICST™

Transduction reagent, Viro-MICST™

Supplier: OZ BIOSCIENCES

Viro-MICST™ transduction reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on i-MICST™ Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection/Transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system. For efficient and selective gene delivery to target cells, Viro-MICST™ can be used in combination with any viral vector and allows you to reduce cell manipulation steps and save time and material.

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Transfection reagent, Insect GeneJuice®

Supplier: Merck Millipore (Novagen)

Insect GeneJuice® Transfection Reagent is formulated to maximise transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for co-transfection of transfer plasmids with linearised virus DNA for the production of recombinant baculoviruses.

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