Tamponi di estrazione

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: OMEGA BIO-TEK

Tampone SP2, Omega Bio-Tek

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Thermo Fisher Scientific

RBC lysis buffer for mouse, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

For cell lysis and protein extraction.

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

Il reagente per estrazione proteica BugBuster®  è formulato espressamente per una lisi delicata della parete cellulare dell'E. coli e per la conseguente liberazione delle proteine solubili. Questo reagente rappresenta un'alternativa semplice, rapida ed economica ai metodi meccanici (frammentazione a pressione 'French Press' o sonicazione) per il rilascio delle proteine bersaglio prima della purificazione o di altre applicazioni. Nella sua formulazione, il reagente utilizza una miscela di detergenti in grado di perforare la parete cellulare senza denaturare le proteine solubili.

Supplier: Thermo Fisher Scientific

RBC lysis buffer for human, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

Supplier: Rockland Immunochemicals

Cell lysis buffer is used to lyse cells under non-denaturing conditions.

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific