Tamponi per ibridazione
I tamponi per elettroforesi mantengono livelli di pH costanti durante la separazione di DNA, RNA e altre proteine. Fornendo ioni che trasferiscono la corrente applicata, le soluzioni a bassa carica facilitano la migrazione di molecole di diverse dimensioni a velocità differenti per l’analisi. Le sostanze preparative offrono protezione aggiuntiva alle proteine, prevenendone la degradazione per garantire la vitalità dei campioni e risultati accurati. Disponibili in vari pesi molecolari, il tampone più adatto può essere selezionato in base alle specifiche macromolecole trattate.
Tampone SSC, 20X, ultrapuro
Supplier: VWR Chemicals
Tampone SSC di ibridazione per Northern e Southern blotting.
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VWR®, Hybridisation buffers with 50% formamide
Supplier: VWR Chemicals
Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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In-situ hybridisation buffer for HPV probes
Supplier: Enzo Life Sciences
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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In situ hybridisation wash reagent
Supplier: Enzo Life Sciences
Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: Enzo Life Sciences
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)
Supplier: G-Biosciences
Saline-sodium phosphate-EDTA hybridisation buffer [20X].
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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North2South™ Hybridisation Buffer
Supplier: Thermo Fisher Scientific
North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.
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Buffer, SSC 20X powder for molecular biology, MP Biomedicals
Supplier: MP Biomedicals
SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.
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Hybridisation buffer, SSPE 20X, AccuGENE™
Supplier: LONZA
AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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