Vettori, plasmidi e librerie
Nella clonazione molecolare, i vettori sono molecole di DNA utilizzate per introdurre artificialmente materiale genetico estraneo in un’altra cellula. I quattro principali tipi di vettori sono plasmidi, vettori virali, cosmidi e cromosomi artificiali, con i plasmidi come i più utilizzati. I vettori possono essere impiegati per la clonazione e alcuni sono specificamente progettati a tale scopo, mentre altri sono destinati alla trascrizione e all’espressione proteica. I vettori di trascrizione più semplici vengono utilizzati per amplificare il materiale genetico inserito.
Human Tagged ORF Clone, GPR172B (SLC52A1)
Supplier: OriGene
Lenti ORF particles, SLC52A1 (mGFP-tagged) - Human G protein-coupled receptor 172B (GPR172B), transcript variant 1, 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, SLC52A3
Supplier: OriGene
Lenti ORF particles, SLC52A3 (mGFP-tagged)-Human chromosome 20 open reading frame 54 (C20orf54), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, SGOL2 (SGO2)
Supplier: OriGene
SGOL2 (Myc-DDK-tagged)-Human shugoshin-like 2 (S. pombe) (SGOL2), transcript variant 1. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone Lentiviral Particle, PD-L1 (CD274)
Supplier: OriGene
Lenti ORF particles, CD274 (Myc-DDK tagged) - Human CD274 molecule (CD274), 200 µl, >10⁷ TU/ml.
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Human Tagged ORF Clone Lentiviral Particle, ICOS Ligand (ICOSLG)
Supplier: OriGene
7 TU/ml.
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Human Tagged ORF Clone, GPCR MRGX2 (MRGPRX2)
Supplier: OriGene
MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2). This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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AAV helper-free system
Supplier: AGILENT
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
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Human Tagged ORF Clone Lentiviral Particle, ICOS
Supplier: OriGene
7 TU/ml.
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Human Tagged ORF Clone, CFTR (NM_000492)
Supplier: OriGene
This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, P Glycoprotein (ABCB1)
Supplier: OriGene
This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1).
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Human Tagged ORF Clone Lentiviral Particle, BRG1 (SMARCA4)
Supplier: OriGene
7 TU/ml.
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Human Tagged ORF Clone, GPR172A (SLC52A2)
Supplier: OriGene
Lenti ORF particles, SLC52A2 (mGFP-tagged) - Human G protein-coupled receptor 172A (GPR172A), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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InterPlay Adenoviral TAP Systems
Supplier: AGILENT
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
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Mouse Untagged Clone Plasmid, Lrrc15 (NM_028973)
Supplier: OriGene
Lrrc15 (untagged) - Mouse leucine rich repeat containing 15 (Lrrc15).
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Mammalian TAP system, interPlay
Supplier: AGILENT
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
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Lambda DNA
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Lambda DNA is a temperate E. coli bacteriophage.
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