Vettori, plasmidi e librerie
Nella clonazione molecolare, i vettori sono molecole di DNA utilizzate per introdurre artificialmente materiale genetico estraneo in un’altra cellula. I quattro principali tipi di vettori sono plasmidi, vettori virali, cosmidi e cromosomi artificiali, con i plasmidi come i più utilizzati. I vettori possono essere impiegati per la clonazione e alcuni sono specificamente progettati a tale scopo, mentre altri sono destinati alla trascrizione e all’espressione proteica. I vettori di trascrizione più semplici vengono utilizzati per amplificare il materiale genetico inserito.
pSTBlue-1 AccepTor™ vector kit
Supplier: Merck Millipore (Novagen)
The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.
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Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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E. coli BL21
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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M13KO7 Helper Phage, Cytiva
Supplier: Cytiva
M13 phage for the GST expression system.
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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors
Supplier: Thermo Fisher Scientific
Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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Expression vectors, green renilla Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis
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Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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Vector, pBR322, MP Biomedicals
Supplier: MP Biomedicals
pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).
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Expression vectors, cypridina Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.
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Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™
Supplier: Thermo Fisher Scientific
Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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Vector, pUC 18, MP Biomedicals
Supplier: MP Biomedicals
pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
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Cell-free protein synthesis, rapid translation system (RTS) vectors
Supplier: biotechrabbit
Expression vectors for PCR-based generation of linear templates.
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck