Vettori, plasmidi e librerie
Nella clonazione molecolare, i vettori sono molecole di DNA utilizzate per introdurre artificialmente materiale genetico estraneo in un’altra cellula. I quattro principali tipi di vettori sono plasmidi, vettori virali, cosmidi e cromosomi artificiali, con i plasmidi come i più utilizzati. I vettori possono essere impiegati per la clonazione e alcuni sono specificamente progettati a tale scopo, mentre altri sono destinati alla trascrizione e all’espressione proteica. I vettori di trascrizione più semplici vengono utilizzati per amplificare il materiale genetico inserito.
Human cDNA Clone
Supplier: OriGene
KCNH2 (untagged) - human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 3
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AdEasy Adenoviral vector systems
Supplier: AGILENT
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
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Vectors, pBlueScript II
Supplier: AGILENT
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
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P2ry2 (NM_008773) Mouse Tagged ORF Clone
Supplier: OriGene
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use.
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Human Untagged LRRC15 Clone
Supplier: OriGene
LRRC15 (untagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2.
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SureVector
Supplier: AGILENT
Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.
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pAAV-AC-GFP AAV Gene Expression Vector
Supplier: OriGene
AAV Gene Expression Vectors with C-terminal tGFP tag.
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Mouse Untagged Clone Plasmid, Lrrc15 (NM_028973)
Supplier: OriGene
Lrrc15 (untagged) - Mouse leucine rich repeat containing 15 (Lrrc15).
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Lambda DNA
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Lambda DNA is a temperate E. coli bacteriophage.
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Mammalian TAP system, interPlay
Supplier: AGILENT
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
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Mouse Tagged ORF Clone, Basp1
Supplier: OriGene
Basp1 mouse tagged ORF clone expression plasmid. Basp1 (Myc-DDK-tagged) - Mouse brain abundant, membrane attached signal protein 1 (Basp1).
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Human shRNA Plasmid Kit, ANKRD55
Supplier: OriGene
Human shRNA plasmid kit, ANKRD55 is designed against multiple splice variants at this gene locus.
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SLK Human shRNA Plasmid Kit (Locus ID 9748)
Supplier: OriGene
These shRNA constructs were designed against multiple splice variants at this gene locus.
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Human Tagged ORF Clone, PSMA (FOLH1) (NM_001014986)
Supplier: OriGene
FOLH1 (Myc-DDK-tagged)-Human folate hydrolase (prostate-specific membrane antigen) 1 (FOLH1), transcript variant 2.
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pBK-CMV phagemid vector
Supplier: AGILENT
pBK-CMV vector used to express proteins in both E. coli and mammalian model systems.
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Human Tagged ORF Clone Lentiviral Particle, CD252 (TNFSF4)
Supplier: OriGene
7 TU/ml.
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