Hai cercato: Enzimi

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: Thermo Fisher Scientific

Proteinase K

Supplier: Merck Millipore (Calbiochem‎)

Bovine Ribonuclease A (from Pancreas)

Supplier: Merck Millipore (Calbiochem‎)

Complex metalloprotein that catalyses oxidative hydroxylation of a variety of aromatic heterocycles and simple aldehydes. Catalyses the oxidation of xanthine to uric acid.

Supplier: Merck Millipore (Calbiochem‎)

Pronase E (from Streptomyces griseus)

Supplier: Merck Millipore (Calbiochem‎)

Pig L-Lactate dehydrogenase (from Heart)

Supplier: OMEGA BIO-TEK

Proteinase K Solution

Supplier: Thermo Fisher Scientific

The Pierce™ DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: EDVOTEK

Proteinase K

Supplier: PanReac AppliChem

Lysozyme (Muramidase)

Supplier: Thermo Fisher Scientific

Lysozyme (Muramidase)

Supplier: Cytiva

A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.

Supplier: Merck Millipore (Oncogene)

Horseradish peroxidase

Supplier: Thermo Fisher Scientific

Yeast lytic enzyme

Supplier: Merck Millipore (Novagen)

Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Supplier: Corning

0,05% Trypsin/0,53 mM EDTA in HBSS (Hank’s Balanced Salt Solution) with sodium bicarbonate, without calcium and magnesium.

Supplier: Merck Millipore (Novagen)

Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Supplier: ProSci Inc.

Xaa-Pro aminopeptidase 2 (XPNPEP2) belongs to the peptidase M24B family of metalloproteases. Human XPNPEP2 is widely expressed in various tissues, such as kidney, lung, heart, placenta, liver, small intestine and colon. However, it doesn’t express in brain, skeletal muscle, pancreas, spleen, thymus, prostate, testis and ovary. XPNPEP2 is a Homotrimer which binds 2 manganese ions per subunit. The metalloprotease XPNPEP2 may play a role in the inflammatory process and other reactions produced in response to injury or infection and the metabolism of the vasodilator bradykinin.

Supplier: ProSci Inc.

Carboxylesterase 5 (CES5), also called cauxin or CES7, is a member of carboxylesterases family which plays an important role in the hydrolysis of ester and amide bonds. Carboxylesterase is a type of enzyme that capable of hydrolysing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. CES5 is with broad substrate specificity ranging from small molecule esters to longchain fatty acid esters and thioesters. It has been previously reported CES5 was in high concentrations in the urine (cauxin) of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for CES5 may include regulating urinary levels of male cat pheromones, catalysing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics.

Supplier: ProSci Inc.

D-tyrosyl-tRNA(Tyr) deacylase 1(DTD1) belongs to the DTD family, and expressed in many adult and fetal tissues such as testis, ovary, spleen in adult and fetal brain. It is a nucleus and cytoplasm located protein, and is preferentially phosphorylated in cells arrested early in S phase. DTD1 is an ATPase involved in DNA replication, it may facilitate loading of CDC45 onto pre-replication complexes. The protein may hydrolyse D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), a possible defense mechanism against a harmful effect of D-tyrosine.

Supplier: ProSci Inc.

L-Xylulose Reductase is an enzyme that belongs to the Short-Chain Dehydrogenases/Reductases (SDR) family. L-Xylulose Reductase is responsible for the metabolism of Xylulose, converting it into Xylitol. L-Xylulose Reductase catalyses the NADPH-dependent reduction of several Pentoses, Tetroses, Trioses, alpha-Dicarbonyl compounds and L-Xylulose. L-Xylulose Reductase participates in the Uronate Cycle of Glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing Xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.

Supplier: ProSci Inc.

Peptidyl-Prolyl Cis-Trans Isomerase-Like 1 (PPIase) belongs to the cyclophilin-type PPIase family. PPIases can accelerate the folding of proteins and catalyze the cis-trans isomerisaion of proline imidic peptide bonds in oligopeptides. PPIase is a ubiquitous protein and has highly expression in heart ,skeletal and muscle. PPIase contains a PPIase cyclophilin-type domain and four Cyclosporin A binding regions. PPIase might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is suggested that PPIase can act as as a novel molecular target for colon-cancer therapy.

Supplier: ProSci Inc.

Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isoforms by alternative splicing. Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased, which Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. SEPHS1 can be activated by phosphate ions and by potassium ions. It can synthesise synthesises selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesise selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.

Supplier: ProSci Inc.

beta Galactosidase is a lysosomal beta Galactosidase that hydrolyses the terminal beta Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature beta Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of beta Galactosidase results a catalytically inactive beta Galactosidase that plays an important role in vascular development. Defects of beta -galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for beta Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.

Supplier: ProSci Inc.

Glycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein. GNMT catalyses the synthesis of N-methylglycine from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. It can affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine, playing an important role in maintaining normal AdoMet levels. GNMT is highly expressed in liver. As a major folate-binding protein, GNMT takes part in the detoxification pathway. Defects in GNMT are the cause of hypermethioninemia. the patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.

Supplier: ProSci Inc.

NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognising either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi.

Supplier: ProSci Inc.

Chymotrypsin-Like Elastase Family Member 3A (CELA3A) is an enzyme that contains one peptidase S1 domain. ELA3A belongs to the peptidase S1 family of the Elastase subfamily. ELA3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. ELA3A may also function in the intestinal transport and metabolism of cholesterol. ELA3A is efficient protease with alanine specificity but only little elastolytic activity. ELA3A preferentially cleaves proteins after alanine residues.