Hai cercato: Enzimi
Enzymes accelerate, or catalyse, chemical reactions, and they are known to catalyse more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Calf alkaline phosphatase (from intestine), Pierce™
Supplier: Thermo Fisher Scientific
This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods. The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA. For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.
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Recombinant Proteinase K
Supplier: PanReac AppliChem
Recombinant Proteinase K
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Penicillinase (beta-Lactamase) (from Bacillus cereus)
Supplier: Merck Millipore (Calbiochem)
Penicillinase (beta-Lactamase) (from Bacillus cereus)
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L-Lactate dehydrogenase (from Muscle)
Supplier: Merck Millipore (Calbiochem)
L-Lactate dehydrogenase (from Muscle)
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Bovine trypsin (from pancreas)
Supplier: Merck Millipore (Calbiochem)
Bovine trypsin (from pancreas)
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Alcalase (from Bacillus licheniformis)
Supplier: Merck Millipore (Calbiochem)
A serine endopeptidase that consists primarily of subtilisin A. Suitable for the hydrolysis of proteins.
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Streptococcus pyogenes Cas9 nuclease
Supplier: OZ BIOSCIENCES
Optimised Cas9 Nuclease S. Pyogenes is designed for genome editing in living cells or organisms and also for in vitro digestion.
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Adenosylhomocysteine nucleosidase
Supplier: G-Biosciences
This recombinant adenosylhomocysteine nucleosidase (10 µM) converts S-adenosylhomocysteine to S-(deoxy-D-ribos-5-yl)-L-homocysteine and adenine.
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Human recombinant caspase 8
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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Pierce™ Horseradish Peroxidase
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish Peroxidase
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Urease (from Canavalia ensiformis)
Supplier: Merck Millipore (Calbiochem)
Native urease from Canavalia ensiformis. Catalyzes hydrolysis of urea to carbon dioxide and ammonia. Useful in the determination of urea in body fluids.
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Merck Millipore (Calbiochem)
Chicken lysozyme (Muramidase) (from egg white)
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Tritirachium album proteinase K
Supplier: PanReac AppliChem
Proteinase K (Protease K) is a broad-spectrum serine protease from Tritirachium album.
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Human recombinant caspase 1
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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Pig Pepsin (from Gastric mucosa)
Supplier: Merck Millipore (Calbiochem)
Pig Pepsin (from Gastric mucosa)
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Bovine trypsin (from pancreas)
Supplier: Merck Millipore (Calbiochem)
Bovine trypsin (from pancreas)
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Human recombinant caspase 6
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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Trypsin digestion mix
Supplier: G-Biosciences
Trypsin digestion mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.
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Trichoderma viride cellulase
Supplier: Merck Millipore (Calbiochem)
Cellulase is an effective enzyme to isolate intact cells and protoplasts from plant tissues.
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Streptomyces sp. N174 chitosanase (from Streptomyces sp. N174)
Supplier: Merck Millipore (Calbiochem)
Chitosanase from Streptomyces species is a new class of enzymes with antifungal properties that efficiently catalyses the hydrolysis of acetylated chitosan.
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Bovine desoxyribonuclease I (from Pancreas)
Supplier: Merck Millipore (Calbiochem)
Bovine desoxyribonuclease I (from Pancreas)
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Glucose oxidase (from Aspergillus niger)
Supplier: Merck Millipore (Oncogene)
Glucose oxidase (from Aspergillus niger)
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Pronase E (from Streptomyces griseus)
Supplier: Merck Millipore (Calbiochem)
Pronase E (from Streptomyces griseus)
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Potato native acid phosphatase
Supplier: Merck Millipore (Calbiochem)
Native potato acid phosphatase. Ideal for the removal of phosphate groups from phosphoproteins, such as β-casein, ovalbumin and pepsinogen.
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Pyruvate oxidase
Supplier: Merck Millipore (Calbiochem)
Pyruvate oxidase from Aerococcus viridans catalyses the oxidation of pyruvate to acetyl phosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.
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Human recombinant caspase 2
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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Human recombinant caspase 7
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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MegaWox™ polyHRP Streptavidin Conjugate (1 mg)
Supplier: AAT BIOQUEST
Immunoassays (such as histochemistry, ELISAs and Western blotting) are important in clinical diagnosis and experimental protein analysis.
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DIG-HRP Conjugate (1 mg)
Supplier: AAT BIOQUEST
Digoxigenin (DIG)-conjugated horse radish peroxidase (HRP) is an excellent tool for biological detections with ELISA and other DIG-coupled assays.