Hai cercato: Enzimi

Supplier: MERCK PRODUCTION CHEMICALS

Pig Pancreatin, Protease, Lipase, Amylase (from Pancreas)

Supplier: MP Biomedicals

Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.

Supplier: MP Biomedicals

Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.

Supplier: MP Biomedicals

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

Supplier: MP Biomedicals

Readily soluble in water.

Supplier: MP Biomedicals

Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis.

Supplier: MP Biomedicals

Suitable for both protein and nucleic acid isolation. Exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine. Useful in the isolation of DNA and RNA, in the analysis of membrane structures and protein structure. Proteolytic activity: >30mAnson Units/mg Unit definition: One Anson unit is the amount of enzyme which liberates 1 µmol of Folin-positive amino acids per minute at pH 7,5 and 35 °C, using hemoglobin as substrate.

Supplier: MP Biomedicals

The hyaluronidase is purified from Streptomyces hyalurolyticus. This enzyme belongs to the endo-beta-hexosaminidase and endo-eliminase type enzyme that produces oligo-saccharide (unsaturated tetraose and pentose) by hydrolysing the beta-1,4-linkage of hyaluronic acid. It is stable in both high temperature and acid or alkaline range. It has high substrate specificity and hydrolyzes hyaluronic acid only in mucopolysaccharide.

Supplier: MP Biomedicals

Proline specific endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues.

Supplier: MP Biomedicals

Dissolve at a concentration of 1 mg/ml. Dilute further to a concentration of 20 to 60 U/ml immediately before the assay.

Supplier: MP Biomedicals

Pig Diaphorase (from Heart)

Supplier: Abnova

Recombinant Vaccinia virus capping enzyme (from E. coli)

Supplier: Abnova

Recombinant Vaccinia virus capping enzyme (from E. coli)

Supplier: Abnova

Recombinant Bovine ENTEROKINASE (from P. pastoris), His-tag

Supplier: Abnova

Collagenases are enzymes that break the peptide bonds in collagen.

Supplier: MP Biomedicals

One unit will hydrolyse 1,0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25 °C and pH 6,2.

Supplier: MERCK PRODUCTION CHEMICALS

Benzonase®

Supplier: MERCK PRODUCTION CHEMICALS

Humectants for confectionery

Supplier: MERCK PRODUCTION CHEMICALS

The best-in-class solution for nucleic acid removal at high salt concentration in Bioprocessing. The Benzonase® Salt Tolerant endonuclease Emprove® Expert is a new enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations.

Supplier: Abnova

Protease

Supplier: Abnova

Recombinant Alkaline Phosphatase (from E. coli)

Supplier: Abnova

Glutathione S-Transferase

Supplier: Abnova

Recombinant Poly(A) Polymerase (from E. coli)

Supplier: MP Biomedicals

Protease isolated from S. aureus, strain V8, this protease exhibits cleavage of peptide bonds on the COOH-terminal side of either aspartate or glutamate residues.

Supplier: MP Biomedicals

Pig Trypsin (from Pancreas)

Supplier: MP Biomedicals

Unit Definition: Enzyme activity is determined by a change in optical density in a solution of hyaluronic acid. Activity is compared to a hyaluronidase International Standard and is expressed as an International Unit (U).

Supplier: MP Biomedicals

Inhibitors: p-Chloromercuribenzoate, iodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).

Supplier: MP Biomedicals

Cellulase Y-C removes cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.

Supplier: MP Biomedicals

Pectolyase Y-23 contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. It includes maceration stimulating factor which stimulates tissue maceration by both pectinases.

Supplier: MP Biomedicals

Elastase is prepared from porcine pancreas. It is lyophilised and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialysed until chloride-free and then lyophilised. During its preparation the elastase is held below a pH of 5,5 for greater than 24 hours. Two times crystallised from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.