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18 results for Reagenti per sequenziamento | Avantor

Reagenti per sequenziamento

Reagenti per sequenziamento

I reagenti di sequenziamento facilitano la determinazione dell’ordine preciso dei nucleotidi nei filamenti di DNA attraverso la separazione dei frammenti in base alla dimensione. Gli enzimi sono ottimizzati per lavorare insieme e rimuovere in modo altamente efficiente i primer e i nucleotidi non incorporati. Questi reagenti producono risultati di sequenziamento eccezionali anche da matrici difficili, come quelle con elevato contenuto di GC e strutture secondarie. Essenziali per la ricerca di base e applicata, i reagenti di sequenziamento consentono ai ricercatori di scoprire i genotipi e i fenotipi dei campioni.

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Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Supplier: Corning

The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.

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MagaDye™ 561 ddATP

MagaDye™ 561 ddATP

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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MagaDye™ 588 ddTTP

MagaDye™ 588 ddTTP

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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2-Aminoethoxypropargyl ddGTP (1 µmole)

2-Aminoethoxypropargyl ddGTP (1 µmole)

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

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MagaDye™ 535 ddGTP

MagaDye™ 535 ddGTP

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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MagaDye™ 613 ddCTP

MagaDye™ 613 ddCTP

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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2-Aminoethoxypropargyl ddCTP (1 µmole)

2-Aminoethoxypropargyl ddCTP (1 µmole)

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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2-Aminoethoxypropargyl ddATP (1 µmole)

2-Aminoethoxypropargyl ddATP (1 µmole)

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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2-Aminoethoxypropargyl ddTTP (1 µmole)

2-Aminoethoxypropargyl ddTTP (1 µmole)

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

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Lysine-C™, sequencing grade

Supplier: G-Biosciences

SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.

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Arginine-C™, sequencing grade

Supplier: G-Biosciences

SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

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Chymotrypsin™, sequencing grade

Supplier: G-Biosciences

SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.

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