Reagenti per sequenziamento
I reagenti di sequenziamento facilitano la determinazione dell’ordine preciso dei nucleotidi nei filamenti di DNA attraverso la separazione dei frammenti in base alla dimensione. Gli enzimi sono ottimizzati per lavorare insieme e rimuovere in modo altamente efficiente i primer e i nucleotidi non incorporati. Questi reagenti producono risultati di sequenziamento eccezionali anche da matrici difficili, come quelle con elevato contenuto di GC e strutture secondarie. Essenziali per la ricerca di base e applicata, i reagenti di sequenziamento consentono ai ricercatori di scoprire i genotipi e i fenotipi dei campioni.
Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit
Supplier: Corning
The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
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MagaDye™ 561 ddATP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 588 ddTTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddGTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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MagaDye™ 613 ddCTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 535 ddGTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddCTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddATP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddTTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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Lysine-C™, sequencing grade
Supplier: G-Biosciences
SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
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Arginine-C™, sequencing grade
Supplier: G-Biosciences
SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
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Chymotrypsin™, sequencing grade
Supplier: G-Biosciences
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck