Reagenti per microscopia e colorazione

Supplier: Merck

Le soluzioni di montaggio non acquose, ad esempio Neo-Mount®, sono destinate al montaggio permanente e alla conservazione a lungo termine dei campioni. I vetrini processati nei laboratori patologici devono essere conservati fino a 10 anni, di conseguenza i risultati della colorazione devono essere stabili e non possono ammettere scolorimenti. La soluzione di montaggio anidra Neo-Mount® per microscopia è estremamente stabile e prodotta con solventi a base di miscele di idrocarburi alifatici. Poiché contiene un componente privo di aromatici in sostituzione dello xilene, la soluzione Neo-Mount® può essere combinata esclusivamente con Neo-Clear®. Non utilizzare lo xilene in fase di montaggio, in quanto causa l'offuscamento e la striatura dei vetrini. In aggiunta, posizionando i vetrini disidratati sulla carta filtrante per circa 1 minuto prima del montaggio, si impedisce l'eventuale eccesso di Neo-Clear®, in quanto sotto il vetrino coprioggetto potrebbero formarsi bolle di aria. Tale azione preventiva è da eseguire anche quando si montano i vetrini con macchine montavetrini; in quest'area, un'efficace eliminazione di NEO-CLEAR® si ottiene incubando i vetrini per un minuto in un rack per vetrini vuoto.

Supplier: Merck

Entellan® new è una nuova soluzione di montaggio per montavetrini ed è particolarmente adatta per strumenti commerciali di montaggio automatizzati utilizzati unitamente a montavetrini. Ha un range di viscosità di 250 - 600 mPas, il cod. prod. 1,00869 ha un range inferiore. Questo basso range di viscosità migliora e accelera l'applicazione, in quanto non occorre ripristinare il tasso di erogazione o il volume dopo il primo ciclo con un nuovo flacone di Entellan® new.

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: VWR Chemicals

Safemount Q Path® è una soluzione di montaggio, fornita in un flacone in PET, compatibile con Safesolv. Consente di incollare il vetrino coprioggetto al vetrino e fissa la sonda in posizione dopo la colorazione.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.