Supplier: OriGene

FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

Supplier: OriGene

Human shRNA plasmid kit, ADHFE1 is designed against multiple splice variants at this gene locus.

Supplier: OriGene

GABA B receptor 2 (GABBR2), human tagged ORF clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Supplier: AGILENT

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

Supplier: AGILENT

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Supplier: AGILENT

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

Supplier: OriGene

LRRC15 (untagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2.

Supplier: OriGene

pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.

Supplier: OriGene

KCNH2 (untagged) - human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 1

Supplier: OriGene

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use.

Supplier: OZ BIOSCIENCES

pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

Supplier: Merck Millipore (Novagen)

The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

Supplier: OriGene

Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).

Supplier: Merck Millipore (Novagen)

pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

Supplier: Thermo Fisher Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Supplier: Thermo Fisher Scientific

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

Supplier: Thermo Fisher Scientific

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Supplier: Cytiva

M13 phage for the GST expression system.

Supplier: Thermo Fisher Scientific

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

Supplier: Thermo Fisher Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.