Vettori, plasmidi e librerie
Nella clonazione molecolare, i vettori sono molecole di DNA utilizzate per introdurre artificialmente materiale genetico estraneo in un’altra cellula. I quattro principali tipi di vettori sono plasmidi, vettori virali, cosmidi e cromosomi artificiali, con i plasmidi come i più utilizzati. I vettori possono essere impiegati per la clonazione e alcuni sono specificamente progettati a tale scopo, mentre altri sono destinati alla trascrizione e all’espressione proteica. I vettori di trascrizione più semplici vengono utilizzati per amplificare il materiale genetico inserito.
Human Tagged ORF Clone, GPR172B (SLC52A1)
Supplier: OriGene
Lenti ORF particles, SLC52A1 (mGFP-tagged) - Human G protein-coupled receptor 172B (GPR172B), transcript variant 1, 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, SLC52A3
Supplier: OriGene
Lenti ORF particles, SLC52A3 (mGFP-tagged)-Human chromosome 20 open reading frame 54 (C20orf54), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, SGOL2 (SGO2)
Supplier: OriGene
SGOL2 (Myc-DDK-tagged)-Human shugoshin-like 2 (S. pombe) (SGOL2), transcript variant 1. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, GPCR MRGX2 (MRGPRX2)
Supplier: OriGene
MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2). This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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AAV helper-free system
Supplier: AGILENT
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
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Human Tagged ORF Clone, CFTR (NM_000492)
Supplier: OriGene
This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone, GPR172A (SLC52A2)
Supplier: OriGene
Lenti ORF particles, SLC52A2 (mGFP-tagged) - Human G protein-coupled receptor 172A (GPR172A), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Tagged ORF Clone Lentiviral Particle, PD-L1 (CD274)
Supplier: OriGene
Lenti ORF particles, CD274 (Myc-DDK tagged) - Human CD274 molecule (CD274), 200 µl, >10⁷ TU/ml.
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Human Tagged ORF Clone Lentiviral Particle, ICOS Ligand (ICOSLG)
Supplier: OriGene
7 TU/ml.
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Human Tagged ORF Clone, P Glycoprotein (ABCB1)
Supplier: OriGene
This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1).
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InterPlay Adenoviral TAP Systems
Supplier: AGILENT
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
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Human cDNA Clone
Supplier: OriGene
KCNH2 (untagged) - human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 3
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AdEasy Adenoviral vector systems
Supplier: AGILENT
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
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Vectors, pBlueScript II
Supplier: AGILENT
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
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