buffers

Supplier: EUROCLONE

Cloning Buffer, TRIS solution tampon pH 7,5 (2 mol/l)

Supplier: Molekula

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 8,0

Supplier: EUROCLONE

Tampon de migration, TAE tampon 10x

Supplier: Molekula

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 7,5

Supplier: Ambion

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 8,0

Supplier: EUROCLONE

Tampon de migration, Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée

Supplier: EUROCLONE

Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

Supplier: EUROCLONE

Tampon d'hybridation, TBS tampon solide pour solution concentrée 20x

Supplier: HORIBA

Tampon pH 7,00 (6 flacons de 14 ml par paquet), Flacon

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

Supplier: EUROCLONE

Tampon de migration, TBE tampon 5X

Supplier: Merck Millipore (Novagen)

Le réactif d'extraction de protéines BugBuster® est formulé de façon à rompre délicatement la paroi cellulaire de l'E. coli, afin de libérer la protéine soluble. Il offre une alternative simple, rapide et économique aux méthodes mécaniques (French Press ou sonication) d'extraction de protéines cibles à des fins de purification ou d'autres applications. La formulation utilise un mélange de détergents capable de perforer la paroi cellulaire sans dénaturer la protéine soluble.

Supplier: EUROCLONE

Tampon de migration, TRIS-glycine-SDS solution tampon 10x concentrée (tampon TGS)

Supplier: Thermo Orion

Réalisez des étalonnages de pH reproductibles et précis avec des tampons pH Orion™.