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Tampons

Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.

 
 

Réactif stabilisateur E.Z.N.A.® RNALock

Supplier: OMEGA BIO-TEK

Le réactif stabilisateur E.Z.N.A.® RNALock est un réactif innovant qui lyse rapidement les cellules et les tissus pour désactiver les RNases à température ambiante. Il suffit d'immerger les échantillons, tels que les cultures cellulaires, les tissus animaux ou les globules blancs, dans le réactif stabilisateur RNALock. L'ARN cellulaire intact sera préservé même à température élevée. Les échantillons peuvent être conservés dans ce réactif pendant plus de 12 mois à -20 °C.

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Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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T-PER™ Tissue Protein Extraction Reagent

T-PER™ Tissue Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

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PBS Solution tampon saline 10x concentrée (tamponnée phosphate)

Supplier: EUROCLONE

Washing buffer, PBS Solution tampon saline 10x concentrée (tamponnée phosphate)

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Subcellular protein fractionation kit for tissues

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Y-PER™ Yeast Protein Extraction Reagent

Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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Technical pH buffer solutions, HI 5000 series

Supplier: Hanna

pH Buffer solution, Valeur pH: 1,00, ±0,01, Température de réference: 25 °C, Bouteille en plastique

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SSC Solution tampon saline 20x concentrée (sodium citrate)

Supplier: LONZA

Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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Mammalian protein extract reagents, M-PER™

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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pH electrode calibration and maintenance solutions kit

pH electrode calibration and maintenance solutions kit

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

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HEPES Solution tampon (1 mol/l), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

HEPES Solution tampon (1 mol/l)

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TISAB III solution (Total Ionic Strength Adjustment tampon) pour le dosage des fluorures

TISAB III solution (Total Ionic Strength Adjustment tampon) pour le dosage des fluorures

Supplier: Mettler - Toledo

TISAB III solution for fluoride determinations

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