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buffers

Tampons

Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.

 
 

TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Buffer, EDTA buffer solution pH 8,0 (0,25 mol/L)

Supplier: EUROCLONE

Cloning Buffer, EDTA buffer solution pH 8,0 (0,25 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: EUROCLONE

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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HeLa Nuclear Extract, Abcam

Supplier: Abcam

Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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Réactif d'extraction de protéines, BugBuster® (sans amine primaire), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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Proteometer-CV fluorescent reagent

Proteometer-CV fluorescent reagent

Supplier: NOVILYTIC

1 vial of Fluorescent reagent for the Proteometer-CV kit specifically formulated for use with Proteometer-CV kit.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer)

Supplier: NATIONAL DIAGNOSTICS

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.

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Proteometer-L fluorescent reagent

Proteometer-L fluorescent reagent

Supplier: NOVILYTIC

7 vials of the Proteometer-L fluorescent reagent specifically formulated for use with Proteometer-L kit.

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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Depyrogenated Dilution Tubes

Depyrogenated Dilution Tubes

Supplier: FUJIFILM WAKO CHEMICALS

These depyrogenated dilution tubes are made of high-quality borosilicate glass depyrogenated at 250 °C.

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Proteometer-L kit part 2/2 reagent (1000 assays)

Proteometer-L kit part 2/2 reagent (1000 assays)

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-L kit.

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Proteometer-CV mobile phase B salts

Proteometer-CV mobile phase B salts

Supplier: NOVILYTIC

1 sealed salt packet specifically formulated for use with Proteometer-CV kit.

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