buffers
Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.
TRIS-glycine Solution tampon 10x concentrée (TG tampon)
Supplier: Thermo Fisher Scientific
Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.
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I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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Solution de chargement de gel (10X)
Supplier: EDVOTEK
1 ml de concentré pour 200 échantillons.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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Tris-Glycine buffer
Supplier: EDVOTEK
For vertical polyacrylamide gel electrophoresis.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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IP lysis buffer, Pierce™
Supplier: Thermo Fisher Scientific
The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.
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TE Solution tampon (TRIS-EDTA Tampon) pH 8,0, Low EDTA for Molecular Biolog
Supplier: PanReac AppliChem
Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 8,0
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Non-animal sera protein blocking Agent, FISH-Blocker™
Supplier: G-Biosciences
FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Tampon de migration, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Western Blot Transfer buffer, 10X, methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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pH buffer solutions
Supplier: Hach
pH buffer standard solution with certificate of analysis, 125 ml, Valeur pH: 9,21, Bouteille en plastique
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Luciferase cell lysis buffer (2X), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Pierce TMT™ Elution Buffer
Supplier: Thermo Fisher Scientific
Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.



