buffers

Supplier: OMEGA BIO-TEK

Tampon P2, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Supplier: PanReac AppliChem

HEPES Solution tampon pH 8 (1 mol/l)

Supplier: PanReac AppliChem

HEPES Solution tampon pH 7,5 (1 mol/l)

Supplier: G-Biosciences

Tampon de migration, Tris-Glycine native gel running buffer

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

Supplier: G-Biosciences

Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.

Supplier: G-Biosciences

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

Supplier: G-Biosciences

Ces tampons et réactifs sont exempts de DNase et RNase; ils s'adressent à la recherche de haut niveau.

Supplier: G-Biosciences

Les tampons de lavage femto TBST™ et femto PBST™ améliorent la sensibilité des immunodosages en réduisant le « lavage » des agents immunologiques ou les complexes immunologiques, un problème commun associé aux tampons PBST et TBST classiques. Les tampons de lavage permettent d'offrir des milieux plus propres, ce qui se traduit par un meilleur rapport signal sur bruit. Ils peuvent être utilisés pour les procédures d'incubation et de lavage.

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: G-Biosciences

The IBS™ buffer is specifically developed for solubilisation of inclusion bodies.