buffers

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Supplier: Thermo Fisher Scientific

These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

Supplier: Thermo Fisher Scientific

These stable peroxide and diethanolamine substrate buffers produce optimised, reliable and convenient stock solutions for HRP and alkaline phosphatase assays detection respectively.

Supplier: Thermo Fisher Scientific

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Supplier: Thermo Fisher Scientific

SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Supplier: Thermo Fisher Scientific

Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Supplier: Thermo Fisher Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

Supplier: Thermo Fisher Scientific

Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

Supplier: Hach

The unit-dose powder pillows assure buffer freshness and eliminate the possibility of contamination. Each powder pillow prepares 50 ml of buffer solutions of respective pH.

Supplier: Hach

These buffers are used in the determination of calcium and magnesium in brine.

Supplier: Hach

These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.

Supplier: Hach

Buffers traceable to IUPAC/NIST pH scale.