Supplier: SIGMA ALDRICH MICROSCOPY

Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Supplier: SIGMA ALDRICH MICROSCOPY

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples. N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

Supplier: SIGMA ALDRICH MICROSCOPY

Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes colour from yellow to blue-green at pH 3,8 to 5,4. The monosodium salt of bromocresol green is highly ionisable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Supplier: SIGMA ALDRICH MICROSCOPY

Il s'agit d'un milieu de montage (qui contient 45% de résine acrylique et 55% de xylènes) utilisé en microscopie. Il s'agit d'un adhésif et d'un conservateur d'échantillon pouvant être utilisé manuellement et dans un équipement de collage de lamelles automatique. Adhésif et produit d'étanchéité à usage spécial pour une utilisation avec les matériaux en verre, non plastiques et d'autres matériaux non réactifs.