365 Résultats pour : "VARLP080520VARI00001"

Supplier: SIGMA ALDRICH MICROSCOPY

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Osmium tetroxide (OsO₄) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.

Supplier: SIGMA ALDRICH MICROSCOPY

Dihydrogéno(éthyl)[4-[4-[éthyl(3-sulfonatobenzyl)amino](4-hydroxy-2-sulfonatobenzhydrylidène]cyclohexa-2,5-diène-1-ylidène](3-sulfonatobenzyl)ammonium, sel de disodium, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been used for the staining of conidia.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC).

Supplier: SIGMA ALDRICH MICROSCOPY

Silver nitrate along with iodine are among several inorganic substances used to induce colour in microscopical preparations. Silver nitrate as a biological stain exploits the strong colors of colloidal metallic silver. Silver nitrate is an important component of the silver staining that is used for the detection of proteins separated by polyacrylamide gel electrophoresis. Silver nitrate is a component of the silver stain kit. It is required for image development in diamine as well as non-diamine silver staining.

Supplier: SIGMA ALDRICH MICROSCOPY

A preweighed vial containing 50 mg o-Dianisidine. o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S (C.l. 27195) is an anionic, very hydrophilic disazo dye. It is also known as fast ponceau 2B. Ponceau S is widely used to stain proteins on gel electropherograms. It is a suitable stain for proteins adsorbed onto nitrocellulose membranes. It also has applications in botanical staining manuals as a stain for sieve plate elements.

Supplier: SIGMA ALDRICH MICROSCOPY

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Supplier: SIGMA ALDRICH MICROSCOPY

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

Supplier: SIGMA ALDRICH MICROSCOPY

Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol Blue sodium salt has been used as a pH indicator in the yeast growth media (YPD, yeast extract peptone dextrose).

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: SIGMA ALDRICH MICROSCOPY

Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

Supplier: SIGMA ALDRICH MICROSCOPY

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Supplier: Merck

The Brilliant cresyl blue solution is used in hematology as reagent for the staining of blood for the subsequent manual counting of blood cells. The regenerative capacity of erythrocytes can be monitored with the ratio of reticulocytes. In addition, the determination of the Substantia granulo-filamentosa (ribonucleoproteins) is possible with fresh, unfixed young erythrocytes (in a supra-vitral staining).

Supplier: SIGMA ALDRICH MICROSCOPY

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Phenol Red sodium salt is widely used as a pH indicator. At a pH of 6,4 and below, phenol red solution will have a yellow colour. At a pH of 8,2 and above, it will have a red colour. It is commonly used in culture media to detect neutral to acidic pH changes.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Supplier: SIGMA ALDRICH MICROSCOPY

Wright stain, a modified romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with wright stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

Supplier: SIGMA ALDRICH MICROSCOPY

Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.

Supplier: SIGMA ALDRICH MICROSCOPY

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol green is a sulfonephthalein dye. It is mainly used for measuring serum albumin. In acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630 nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 3.8) to blue color (5.4). It is widely used as a colloidal pigment.

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Blue R has been used in staining of gels after electrophoresis. It has also been used to determine protein in the protein bodies. For detection of protein bands following electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.