1070 Résultats pour : "SIGMA ALDRICH MICROSCOPY"

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Hematein, for microscopy (Hist.).

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(Ammoniated ruthenium oxychloride)

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When immobilised using an insoluble porous support matrix such as agarose, Cibacron Blue 3GA is used for affinity chromatography purification of enzymes. This affinity has been attributed to a structural similarity between the dye and the natural ligands for proteins with cofactor binding domains.

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Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

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Histology slides with paraffin embedded section of human intestine containing argentaffin granules. Suitable for use with Fontana-Masson stain kit (Procedure No. HT200).

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Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Fluorescent probe for hydrophobic (non-polar) regions in proteins and nucleoproteins.

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Lissamine Green B has been used in blood-brain barrier (BBB) studies and is used to study the its effect on herpes simplex virus 1 (HSV-1) corneal infection in animals.

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Dye content - ≥85%.

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Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

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Intended for use in the Gram Stain Procedure No. HT90.

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Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

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3,3′-dihexyloxacarbocyanine iodide (DiOC6) is a supravital lipophilic fluorochrome. This cationic dye is employed to stain mitochondria and endoplasmic reticulum in animal and plant cells. In addition, it acts as a very rapid, consistent and highly selective fluorochrome for endophytic ascomycetes in plant roots and liverworts. DiOC6 also facilitates the detection of different mitochondrial changes happening during early apoptosis.

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Used in procedure HT100 for the toning of sections stained with the silver methanamine procedure.

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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

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Orange G (certified), 0.3% and phosphotungstic acid, 0.015%, in denatured alcohol

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Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Fast Blue RR has been used to detect alkaline phosphatase activity.

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2-Méthoxy-4-nitrophénol 97%, Sigma-Aldrich®

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Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation. It is used as a biomarker in oral rabies vaccine.

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3,3'-Dipropylthiacarbocyanine iodide,98% (3-Propyl-2-[3-[3-propyl-2(3H)benzothiazolylidene]-1-propenyl]benzothiazolium iodide; DiSC3(3))

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Suitable for use as a protease substrate.

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Jenner's stain, suitable for blood stain (Eosin-Methylene Blue)

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5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea).

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Thionin(acetate), Sigma-Aldrich®

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Spin trap used in conjunction with Fe²⁺ to detect NO in brain, kidney, liver, and other tissues. Simultaneous direct measurement of NO* and pO₂ has been reported. A copper chelator, it has been used to quantitate copper in biological materials by ESR and is an inhibitor of superoxide dismutase, ascorbate oxidase, and other enzymes. Also used for colorimetric determination of small quantities of arsenic. Silver diethyldithiocarbamate has been used in cell culture. Also used for colorimetric determination of small quantities of arsenic. Spin trap used in conjunction with Fe²⁺ to detect NO in brain, kidney, liver, and other tissues. Simultaneous direct measurement of NO* and pO₂ has been reported. A copper chelator, it has been used to quantitate copper in biological materials by ESR and is an inhibitor of superoxide dismutase, ascorbate oxidase, and other enzymes.

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A cell-permeable generator of singlet oxygen.

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4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.

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o-Cresolphthaleine, Sigma-Aldrich®

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For use in Sigma Procedure 91C for the demonstration of specific esterase in blood, bone marrow films, tissue touch preparations, cytocentrifuge preparations and paraffin tissue sections.