1070 Résultats pour : "SIGMA ALDRICH MICROSCOPY"

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2,7,12,17-Tetra-tert-butyl-5,10,15,20-tetraaza-21H,23H-porphine has been used in multifunctional drug delivery investigations from ionic triblock terpolymer micelles.

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3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, also known as Ferene, is commonly used to measure the concentration of iron ions in aqueous solutions. The reagent is sensitive to ferrous iron ions (Fe2+) and forms a purple-colored complex with these ions in the presence of a reducing agent. The intensity of the purple color formed is proportional to the concentration of iron ions present in a solution and is quantified spectrophotometrically.

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Citrate Solution for the preparation of a 60% citrate buffered acetone solution used as a fixative for blood smears. No dilution required. Add 30 ml citrate solution to 20 ml absolute acetone for preparation of fixative.

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Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

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Brilliant cresyl blue is a phenoxazine dye.

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Tetramethylrhodamine methyl ester perchlorate (TMRM) is a fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.

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For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

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Kit component in the Masson Trichrome procedure, catalog number HT15

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Acide picrique 13 g/l solution saturée dans le eau, Sigma-Aldrich®

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7-Amino-4-(trifluoromethyl)coumarin (AFC) is a novel fluorescent marker used for the detection of proteinases. Suitable as laser dye.

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SynaptoRed™ C2 has been used for propidium iodide (PI) blockage.

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Vert brillant ≥90% (teneur en colorant), certifié par la Commission de coloration biologique, Sigma-Aldrich®

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Crystal Violet lactone, Dye content 97%.

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10-(2',4'-Dinitrophenylazo)-9-phenanthrol, (DNAF)

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Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid. Thioflavin T has been used in histology and for protein characterization.

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4-(4-Isothiocyanatophenylazo)-N,N-dimethylaniline,97% (4-(Dimethylamino)azobenzene-4'-isothiocyanate; DABITC)

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Phycobiliprotein exhibiting exceptional absorbance and fluorescence characteristics due to a lack of susceptibility to internal and external fluorescence quenching. Ideal for highly sensitive studies in flow cytometry and immunoassays.

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Ethyl 3,5-dimethyl-2-pyrrolecarboxylate,98%

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Acid Phosphatase, Lymphocyte Kit, Sigma-Aldrich®

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Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.

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Orange G solution, alcoholic, for the colorimetric method according to Papanicolaou (OG-6)

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Auramine O, Sigma-Aldrich®

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Yellow counterstain suitable for a wide range of histological staining procedures.

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Suitable for titration of morpholine

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Fast Blue BB Salt hemi (zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt. It has been used for alkaline phosphatase staining in cells.

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1-Aminoanthraquinone has the ability to increase the solubility of derivatives of anthraquinone in supercritical carbon dioxide.

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DNA, RNA stain with advantageous spectral properties for cytometric applications.

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Intended for use in the Leukocyte Acid Phosphatase procedure, procedure No. 386 or 387

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CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.