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Silver Protein

Supplier: SIGMA ALDRICH MICROSCOPY

Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.

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o-Tolidine ≥95%

Supplier: SIGMA ALDRICH MICROSCOPY

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

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Bleu de méthylène solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

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Naphthol AS-GR phosphate disodium salt

Supplier: SIGMA ALDRICH MICROSCOPY

Produces highly insoluble dark green stain in histochemical studies.

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Percoll®, Siam

Supplier: SIGMA ALDRICH MICROSCOPY

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

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Azure A eosinate, poudre, cristaux ou morceaux, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure A eosinate, poudre, cristaux ou morceaux, Sigma-Aldrich®

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2-Amino-5,6-dimethylbenzimidazole, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2-Amino-5,6-dimethylbenzimidazole, Sigma-Aldrich®

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Violet cristallisé solution Grams pour la microscopie, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

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Nile blue chloride (Basic blue 12) 85% (teneur en colorant), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

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Tartrazine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Yellow counterstain suitable for a wide range of histological staining procedures.

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Reactive black 5 ≥50 % (teneur en colorant), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Reactive Black 5 (RB5) has been: used in an optimization study on the photocatalytic degradation of RB5 dye in synthetic greywater effluent (i.e. aqueous RB5 in distilled water), in order to understand the effects of different photoreactor parameters on the degradation kinetics. As a textile dye to investigate the anaerobic reduction in upflow stirred packed-bed reactors (USPBRs) with biological activated carbon (BAC) system. Used in the performance evaluation of dye removal forlarge molecule substance.

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Papanicolaou's solution EA 65, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.

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Silver methenamine

Silver methenamine

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the silver stain (modified GMS) procedure.

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Violet de méthyle 2B pour la microscopie, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl violet for microscopy (Bact., Bot., Hist.), indicator (pH 0.1 - 2.0), mixture of polymethylated pararosaniline hydrochlorides.

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Carmin, en poudre, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carmine is a red compound and is generally used with aluminum, iron and other metal salts to enhance its activity. During smear preparation, it is required for chromatin staining in fresh material. In presence of aluminum chloride, it is used for the staining of glycogen.

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Rouge neutre solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a red nuclear counterstain in various cytochemical procedures.

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Quinoline yellow sulphonic acid sodium salt (Acid yellow 3), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Quinoline Yellow, Mixture of the mono- and disulfonic acids of Quinoline Yellow (Acid Yellow 3)

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FURA-2 pentapotassium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cell impermeable fluorescent probe for Ca2+.

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Solvent Blue 38, "Practical", Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Solvent Blue 38 is a phthalocyanine stain, used for staining myelin, neurons and glial fibres.

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Acridine orange base ≥75% (teneur en colorant), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

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Giemsa colorant, certifié par la Commission de coloration biologique, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

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bisbenzimide H-33342 trihydrochloride (Hoechst 33342) ≥98% (par HPLC, TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic.

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Chlorure de 9-[2-(ethoxycarbonyl)phényl]-3,6-bis(éthylamino)-2,7-diméthylxanthylium ~95% (teneur en colorant), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine 6G has been used as a leukocyte marker to study the interaction between leukocytes and the vascular endothelium. It has also been used to label platelets.

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Pyronine G, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pyronine G, Sigma-Aldrich®

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Fluorescent brightener 28, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps.

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Fast Red Violet LB Salt ≥90% (teneur en colorant), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast Red Violet LB Salt has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.

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Pourpre de bromocresol sel de sodium, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pourpre de bromocresol sel de sodium, Sigma-Aldrich®

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Direct yellow 27, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

As a direct dye, Direct Yellow 27 is an acid dye with large molecules that will bind to cellulose fibers directly. It is mono azo dye with good light fastness.

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Acide 2,7-(bis(2-arsonophénylazo))-1,8-dihydroxynaphtalène-3,6-disulfonique pour détermination du calcium, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

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N-(Diphenylmethylene)glycine ethyl ester 98%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Schiff base which can be converted to amino alcohols and amino esters.

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