"PCR Mastermixes"

Supplier: Thermo Fisher Scientific

Le mélange ABsolute Blue™ incorpore un colorant bleu inerte qui permet d'améliorer considérablement le contraste entre le réactif et le plastique. De ce fait, la vérification de distribution du mélange est rapide, facile et exempte d'anomalies. Les applications incluent l'expression génétique, la validation du siRNA et le génotypage des snip.

Supplier: Quantabio

Ce SuperMix est un cocktail de réaction doublement concentré, prêt à l'emploi pour le qPCR en temps réel qui contient tous les composants mis à part les amorces, les sondes et les modèles. Le système transcende les limites de multiplexage des principaux mélanges PCR conventionnels, assurant une amplification non biaisée d'un maximum de cinq séquences cibles dans un tube unique. La suppression des amplicons à faible nombre de copies par des cibles de référence à grand nombre de copies dans l'amplification est un problème courant dans le multiplexage de PCR. Cela peut fausser ou masquer la représentation visible et la quantification des séquences cibles à faible nombre de copies. Le supermix PerfeCTa® MultiPlex qPCR SuperMix, low ROX fournit une plage dynamique et une sensibilité à la qPCR multiplexée qui est comparable à celle des tests de sonde de qPCR uniplexée sans avoir besoin de concentrations limitées ou variables d'amorce.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

The Real-Time PCR screening kit is addressing three different targets: 35S promoter; NOS Terminator; CTP2-CP4 EPSPS. The tests are all in accordance with the JRC guideline. Advantage of the CTP2-CP4 EPSPS compared to FMV detection is that the used target includes artificial sequences, which are not present naturally like the virus sequence of FMV.

Supplier: Quantabio

La synthèse de l'ADN complémentaire et l'amplification PCR sont réalisées dans le même tube sans l'ouvrir entre les procédures. Ces kits sont optimisés pour fournir une efficacité et une spécificité maximales de la RT-PCR, ce qui permet une co-amplification non biaisée de transcripts à faible nombre de copies en présence de gènes de référence à plus grand nombre de copies. Les kits qScript™ One-Step qRT-PCR offrent donc une meilleure sensibilité que les réactifs de qPCR disponibles sur le marché. Le qScript™ One-Step Master Mix optimise les activités de transcriptase inverse et des enzymes de PCR, tout en réduisant la formation de dimères d'amorce. L'ADN-polymérase AccuStart™ Taq fournit une spécificité élevée grâce à sa liaison aux anticorps monoclonaux pour un meilleur démarrage à chaud. Lorsqu'ils sont chauffés à 95 °C, les anticorps se dénaturent de manière irréversible et libèrent l'ADN polymérase Taq entièrement active et non modifiée. Les kits qScript™ One-Step qRT-PCR sont disponibles pour toutes les plates-formes d'instrument PCR en temps réel, y compris celles nécessitant une normalisation avec le colorant de référence ROX.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Vibrio vulnificus causes severe wound infections and is harmful when ingested. 33% of patients being infected die. The detection in food and water samples using this Real-Time PCR Test is very rapid and versatile. You have results available already after 24 hours. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. It also includes a test for Shigella. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Kit for Vibrio cholerae tox gene (ctx). Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for Vibrio cholerae for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Vibrio parahaemolyticus tox gene (tdh/trh): Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for Vibrio parahaemolyticus for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae . With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research 'VibrioNet' with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-19. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research “VibrioNet” with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-50. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Thermo Fisher Scientific

Le système qRT-PCR Verso™ associe l'enzyme RT Verso™ avec le mélange Master Mix qPCR bleu ABsolute™ pour une commodité et une polyvalence exceptionnelles. Disponible en format 1 ou 2 étapes, ainsi qu'en divers conditionnements, pour toutes vos applications.