"PCR Mastermixes"

Supplier: Enzo Life Sciences

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® est un mélange prêt à l'emploi, hautement sensible pour transcription PCR (RT-qPCR) inverse quantitative des modèles d'ARN.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Merck Millipore (Novagen)

Le NovaTaq PCR Master Mix est un mélange concentré 2X prêt à l'emploi d'ADN polymérase NovaTaq, de désoxyribonucléosides ultrapurs et de tampon de réaction sans chlorure de magnésium. Le Master Mix simplifie la réalisation de réactions PCR et assure des gain de temps, une uniformité accrue et un risque de contamination minimal. Il suffit de mélanger le NovaTaq PCR Master Mix avec un volume égal contenant la quantité requise de chlorure de magnésium, de matrice ADN et d'amorces et la réaction est prête pour le cyclage thermique. La réaction diluée finale contient 2,5 unités d'ADN polymérase NovaTaq par 100 µl. Contient suffisamment de composants pour 200 réactions d'amplification standard de 50 µl (ou 100×100 µl).

Supplier: Q BIOANALYTIC

Real-Time kit for the detection of Staphylococcus aureus.