552 Résultats pour : "Merck Millipore (Novagen)"

Supplier: Merck Millipore (Novagen)

Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Supplier: Merck Millipore (Novagen)

THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Supplier: Merck Millipore (Novagen)

Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: Merck Millipore (Novagen)

BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Supplier: Merck Millipore (Novagen)

Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Supplier: Merck Millipore (Novagen)

The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.

Supplier: Merck Millipore (Novagen)

Le réactif de transfection Insect GeneJuice® est formulé pour optimiser l'efficacité de la transfection des cellules d'insectes Sf9. Le réactif apporte une toxicité extrêmement faible aux cellules et peut être utilisé pour les transfections transitoires et stables dans les milieux avec ou sans sérum, et pour la cotransfection des plasmides de transfert avec ADN de virus linéarisé pour la production de baculovirus recombinés.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: Merck Millipore (Novagen)

Veggie yeast extract is a certified animal-free media component.

Supplier: Merck Millipore (Novagen)

Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

Le NovaTaq PCR Master Mix est un mélange concentré 2X prêt à l'emploi d'ADN polymérase NovaTaq, de désoxyribonucléosides ultrapurs et de tampon de réaction sans chlorure de magnésium. Le Master Mix simplifie la réalisation de réactions PCR et assure des gain de temps, une uniformité accrue et un risque de contamination minimal. Il suffit de mélanger le NovaTaq PCR Master Mix avec un volume égal contenant la quantité requise de chlorure de magnésium, de matrice ADN et d'amorces et la réaction est prête pour le cyclage thermique. La réaction diluée finale contient 2,5 unités d'ADN polymérase NovaTaq par 100 µl. Contient suffisamment de composants pour 200 réactions d'amplification standard de 50 µl (ou 100×100 µl).

Supplier: Merck Millipore (Novagen)

Proteinase K

Supplier: Merck Millipore (Novagen)

Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

Supplier: Merck Millipore (Novagen)

rLysozyme™ solution contains a highly purified and stabilised recombinant lysozyme that can be used for lysis of gram negative bacteria, such as E. coli.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

Supplier: Merck Millipore (Novagen)

Overnight Express™ Autoinduction NMR Medium, 5 L kit

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

Supplier: Merck Millipore (Novagen)

Les marqueurs PCR contiennent un mélange de huit fragments d'ADN définis de 50 à 2 000 bp à des intervalles pratiques pour la caractérisation de petits produits d'ADN. Les marqueurs sont fournis dans un tampon de dissociation contenant deux colorants de traçage qui n'interfèrent en rien avec l'illumination UV des bandes colorées au bromure d'éthidium. Inclus : fiole de tampon de dissociation 6X. Chaque fiole de marqueur est suffisante pour 50 voies d'agarose TBE ou TAE colorées au bromure d'éthidium. La quantité recommandée pour la dissociation (5 µl) donne des bandes d'intensité uniforme, lumineuses, nettes et faciles ? photographier.