552 Results for: "Merck Millipore (Novagen)"

Supplier: Merck Millipore (Novagen)

Le kit BugBuster® Plus Lysonase™ inclut le réactif d'extraction de protéines BugBuster et le réactif de biotraitement Lysonase, un mélange optimisé et prêt à l'emploi de rLysozyme™ et de nucléase Benzonase®. Cette puissante association de deux réactifs permet une récupération maximale des protéines solubles actives dans les bactéries à Gram négatif et à Gram positif. Les réactifs BugBuster et rLysozyme lysent efficacement les cellules bactériennes tandis que la Benzonase dégrade les acides nucléiques pour faciliter le traitement en aval. Requiert 5 ml de BugBuster et 10 µl de Lysonase par gramme de pâte cellulaire. Les kits fournissent suffisamment de réactifs pour l'extraction de protéines dans 20 ou 100 g de pâte cellulaire.

Supplier: Merck Millipore (Novagen)

Le réactif d'extraction de protéines BugBuster HT associe le réactif d'extraction de protéines BugBuster et la nucléase Benzonase® dans un réactif pratique. Le réactif BugBuster HT écarte les problèmes de biotraitement courants dus aux procédures traditionnelles de lyse des cellules. Les protéines solubles sont extraites en douceur de l'E. coli, sans exposition à la chaleur ni stress oxydatif, et la viscosité est éliminée par la digestion des acides nucléiques en une seule étape. L'extrait de protéines qui en résulte peut être facilement fractionné par les techniques de purification classiques. BugBuster HT est parfaitement adapté aux applications de purification des protéines à haut rendement.

Supplier: Merck Millipore (Novagen)

NanoJuice™ Core transfection reagent is a polycationic liposomal which is designed to be used with the NanoJuice™ Transfection Booster. It is highly flammable.

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

Supplier: Merck Millipore (Novagen)

S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

Supplier: Merck Millipore (Novagen)

Les dialyseurs D-tube96™ permettent une dialyse simultanée pratique et à haut rendement de 96 échantillons. Le dispositif offre les avantages du dialyseur Mini D-Tube™ dans un format de 96 tubes. Les dialyseurs D-Tube sont des tubes faciles à manipuler comportant une double membrane fournissant une grande surface pour des dialyses rapides et efficaces. La membrane est ultra-propre, traitée à l'EDTA, en cellulose régénérée exempte de soufre et de métaux lourds.

Supplier: Merck Millipore (Novagen)

Convenient solution for selective degradation of RNA.

Supplier: Merck Millipore (Novagen)

ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

Supplier: Merck Millipore (Novagen)

The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

Supplier: Merck Millipore (Novagen)

Le réactif de transfection GeneJuice® est formulé pour maximiser l'efficacité de transfection, optimiser la facilité d'utilisation et minimiser la cytotoxicité. Il peut être utilisé en alternative à d'autres techniques, telles que la coprécipitation du phosphate de calcium, l'électroporation, la microinjection, la biolistique et la formation de complexes avec le DEAE-dextran.

Supplier: Merck Millipore (Novagen)

D-Tube™ dialyser, D-Tube™ electroelution accessory kit

Supplier: Merck Millipore (Novagen)

Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

Supplier: Merck Millipore (Novagen)

Le kit SpinPrep™ Gel ADN permet l'extraction efficace de fragments d'ADN de 150 bp à >12 000 bp à partir de gels d'agarose. La procédure utilise une solution GelMelt™ pour dissoudre la plaque de gel, avant l'adsorption de l'ADN par une membrane de silice dans un format de colonne de centrifugation. Après une étape de lavage, l'ADN purifié est élué dans du tampon de faible concentration en sels. Chaque colonne de centrifugation peut lier jusqu'à 20 µg d'ADN. Les récupérations sont toujours comprises entre 50 et 90% dans des conditions standard.

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Supplier: Merck Millipore (Novagen)

THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Supplier: Merck Millipore (Novagen)

Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: Merck Millipore (Novagen)

BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Supplier: Merck Millipore (Novagen)

Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Supplier: Merck Millipore (Novagen)

The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.